| An in vitro model of warm hypoxia-reoxygenation injury in human liver endothelial cells. | |
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MedLine Citation:
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PMID: 22472696 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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BACKGROUND: Ischemia-reperfusion or hypoxia-reoxygenation (H-R) injury adversely affects hepatic function following transplantation and major resection; the death of human sinusoidal endothelial cells (SECs) by apoptosis may play a central role in this process. Caspase-3 is an important intracellular protease in the intrinsic and extrinsic pathways of apoptosis. MATERIALS AND METHODS: SECs and EAhy926 cells were exposed to warm hypoxia at 37°C, followed by reoxygenation at 37°C. Activity of caspase-3 was quantified using Western blotting and colorimetric kinase assays. RESULTS: H-R caused a significant increase in caspase-3 activity compared with controls in both cell types. CONCLUSIONS: Warm H-R injury causes apoptotic cell death of SECs and immortalized cells, but with differing patterns of caspase activity. |
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Authors:
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Neal R Banga; K Raj Prasad; J Lance Burn; Shervanthi Homer-Vanniasinkam; Anne Graham |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-3-22 |
Journal Detail:
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Title: The Journal of surgical research Volume: - ISSN: 1095-8673 ISO Abbreviation: - Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-4-4 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376340 Medline TA: J Surg Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Hepatobiliary Surgery and Transplantation, St. James' University Hospital, Leeds, United Kingdom. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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