| A model of sequential heart and composite tissue allotransplant in rats. | |
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MedLine Citation:
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PMID: 20595859 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Some of the 600,000 patients with solid organ allotransplants need reconstruction with a composite tissue allotransplant, such as the hand, abdominal wall, or face. The aim of this study was to develop a rat model for assessing the effects of a secondary composite tissue allotransplant on a primary heart allotransplant. METHODS: Hearts of Wistar Kyoto rats were harvested and transplanted heterotopically to the neck of recipient Fisher 344 rats. The anastomoses were performed between the donor brachiocephalic artery and the recipient left common carotid artery, and between the donor pulmonary artery and the recipient external jugular vein. Recipients received cyclosporine A for 10 days only. Heart rate was assessed noninvasively. The sequential composite tissue allotransplant consisted of a 3 x 3-cm abdominal musculocutaneous flap harvested from Lewis rats and transplanted to the abdomen of the heart allotransplant recipients. The abdominal flap vessels were connected to the femoral vessels. No further immunosuppression was administered following the composite tissue allotransplant. Ten days after composite tissue allotransplantation, rejection of the heart and abdominal flap was assessed histologically. RESULTS: The rat survival rate of the two-stage transplant surgery was 80 percent. The transplanted heart rate decreased from 150 +/- 22 beats per minute immediately after transplant to 83 +/- 12 beats per minute on day 20 (10 days after stopping immunosuppression). CONCLUSIONS: This sequential allotransplant model is technically demanding. It will facilitate investigation of the effects of a secondary composite tissue allotransplant following primary solid organ transplantation and could be useful in developing future immunotherapeutic strategies. |
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Authors:
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Jun Yang; Detlev Erdmann; J C Chang; Issei Komatsu; YiXin Zhang; DanRu Wang; Michael S Hodavance; Scott T Hollenbeck; Howard Levinson; Bruce Klitzman; L S Levin |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Plastic and reconstructive surgery Volume: 126 ISSN: 1529-4242 ISO Abbreviation: Plast. Reconstr. Surg. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-07-02 Completed Date: 2010-09-02 Revised Date: 2011-03-02 |
Medline Journal Info:
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Nlm Unique ID: 1306050 Medline TA: Plast Reconstr Surg Country: United States |
Other Details:
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Languages: eng Pagination: 80-6 Citation Subset: AIM; IM |
Affiliation:
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Division of Plastic, Reconstructive, Maxillofacial, and Oral Surgery, Duke University Medical Center, Durham NC, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Disease Models, Animal Graft Rejection / mortality, prevention & control Graft Survival Heart Rate Heart Transplantation / methods*, mortality Immunosuppressive Agents / therapeutic use Male Neck Rats Rats, Inbred F344 Rats, Inbred WKY Skin Transplantation / methods Surgical Flaps / blood supply* Survival Rate Thoracic Wall / surgery* Transplantation, Heterotopic / methods*, mortality Transplantation, Homologous Treatment Outcome |
| Chemical | |
Reg. No./Substance:
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0/Immunosuppressive Agents |
| Comments/Corrections | |
Comment In:
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Plast Reconstr Surg. 2011 Feb;127(2):1021-2
[PMID:
21285847
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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