| A model for ex vivo spinal cord segment culture--a tool for analysis of injury repair strategies. | |
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MedLine Citation:
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PMID: 20654650 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Most spinal cord injury research is undertaken using in vivo animal models but the extensive care associated with spinalized animals, inherent variability between animals, and complex surgeries makes alternative models especially valuable. Here we present a novel ex vivo model that enables culture of intact post-natal spinal cord segments for up to five days and the assessment of peripheral nerve grafting repair, enhanced with connexin43 antisense oligodeoxynucleotides (Cx43 AsODN), in this model. Down-regulating Cx43 expression with Cx43 AsODN in cultured spinal cord segments prevents cell death and inhibits inflammation spreading from the site of injury to neighbouring tissue, hence maintaining culture viability. Reduction in segment swelling and improvement in neuron survival were evident after Cx43 AsODN treatment. Furthermore, the combination of Cx43 AsODN with peripheral nerve graft implants into cultured spinal cords promoted axon sprouting from the spinal cord into the peripheral nerve graft. This ex vivo spinal cord segment culture model provides a valuable addition to tools currently available for spinal cord injury research. |
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Authors:
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Jie Zhang; Simon J O'Carroll; Ann Wu; Louise F B Nicholson; Colin R Green |
Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-21 |
Journal Detail:
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Title: Journal of neuroscience methods Volume: 192 ISSN: 1872-678X ISO Abbreviation: J. Neurosci. Methods Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-06 Completed Date: 2010-12-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7905558 Medline TA: J Neurosci Methods Country: Netherlands |
Other Details:
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Languages: eng Pagination: 49-57 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Ophthalmology, Faculty of Medical and Health Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand. jie.zhang@auckland.ac.nz |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Axons / drug effects, physiology Cell Survival / drug effects Connexin 43 / metabolism Dose-Response Relationship, Drug Edema / drug therapy, pathology Gene Expression Regulation / drug effects Nerve Regeneration / drug effects, physiology* Neurites / drug effects Neurofilament Proteins / metabolism Neurons / cytology, drug effects* Oligodeoxyribonucleotides, Antisense / pharmacology Organ Culture Techniques Rats Sciatic Nerve / cytology, transplantation* Spinal Cord / cytology, physiology*, surgery* |
| Chemical | |
Reg. No./Substance:
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0/Connexin 43; 0/Neurofilament Proteins; 0/Oligodeoxyribonucleotides, Antisense; 108688-71-7/neurofilament protein H |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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