Document Detail

A model for cystic endometrial hyperplasia/pyometra complex in the bitch.
MedLine Citation:
PMID:  16620926     Owner:  NLM     Status:  MEDLINE    
The objective of this study was to develop a reliable model for the study of the cystic endometrial hyperplasia and pyometra complex (CEH/P) in the bitch. Greyhound bitches (n = 15) were ovariectomised and allocated into three groups (Group 1, n = 5; Group 2, n = 5; Group 3, n = 10, including 5 used from Group 1). Simulated proestrus, estrus and diestrus were induced by treatment with estradiol benzoate and megestrol acetate. The duration of cervical opening during estrus was determined by the intra-vaginal infusion of radio-opaque medium and subsequent radiography of the uterus (Group 1). One milliliter of a culture of Escherichia coli (with five uro-pathogenic virulence factors as identified by PCR: pap, sfa, hlyA, cnf1 and fim) was inoculated intra-vaginally daily throughout the simulated estrus (Group 2). One milliliter of the culture (n = 6) or sterile Luria-Bertani broth (n = 4) was introduced directly into the uterus on simulated diestrus Days 8 or 12 (Group 3). Necropsies were performed 12 and 7-14 days after the inoculation (Groups 2 and 3). The cervix remained open throughout the duration of simulated estrus (5-6 days) in four out of five bitches, and for a shorter duration (3 days of a 6-day estrus period) in one bitch (Group 1). CEH/P was induced by inoculation of bacteria into the uterus (10/10 bitches) but not into the vagina (0/5 bitches), (P = 0.003). A model for the study of CEH/P has been validated.
N Arora; J Sandford; G F Browning; J R Sandy; P J Wright
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Publication Detail:
Type:  Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't     Date:  2006-04-18
Journal Detail:
Title:  Theriogenology     Volume:  66     ISSN:  0093-691X     ISO Abbreviation:  Theriogenology     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-09-18     Completed Date:  2006-11-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1530-6     Citation Subset:  IM    
Department of Veterinary Science, The University of Melbourne, Werribee 3030, Vic., Australia.
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MeSH Terms
Cervix Uteri / physiology
Colony Count, Microbial
Contrast Media
Dog Diseases / microbiology*,  pathology*
Endometrial Hyperplasia / microbiology,  pathology,  radiography,  veterinary*
Escherichia coli / growth & development*
Escherichia coli Infections / microbiology,  pathology,  veterinary*
Estrous Cycle / drug effects,  physiology
Ovariectomy / veterinary
Progesterone / blood
Uterine Diseases / microbiology,  pathology*,  veterinary*
Vaginal Smears
Reg. No./Substance:
0/Contrast Media; 57-83-0/Progesterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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