| A model for cystic endometrial hyperplasia/pyometra complex in the bitch. | |
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MedLine Citation:
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PMID: 16620926 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The objective of this study was to develop a reliable model for the study of the cystic endometrial hyperplasia and pyometra complex (CEH/P) in the bitch. Greyhound bitches (n = 15) were ovariectomised and allocated into three groups (Group 1, n = 5; Group 2, n = 5; Group 3, n = 10, including 5 used from Group 1). Simulated proestrus, estrus and diestrus were induced by treatment with estradiol benzoate and megestrol acetate. The duration of cervical opening during estrus was determined by the intra-vaginal infusion of radio-opaque medium and subsequent radiography of the uterus (Group 1). One milliliter of a culture of Escherichia coli (with five uro-pathogenic virulence factors as identified by PCR: pap, sfa, hlyA, cnf1 and fim) was inoculated intra-vaginally daily throughout the simulated estrus (Group 2). One milliliter of the culture (n = 6) or sterile Luria-Bertani broth (n = 4) was introduced directly into the uterus on simulated diestrus Days 8 or 12 (Group 3). Necropsies were performed 12 and 7-14 days after the inoculation (Groups 2 and 3). The cervix remained open throughout the duration of simulated estrus (5-6 days) in four out of five bitches, and for a shorter duration (3 days of a 6-day estrus period) in one bitch (Group 1). CEH/P was induced by inoculation of bacteria into the uterus (10/10 bitches) but not into the vagina (0/5 bitches), (P = 0.003). A model for the study of CEH/P has been validated. |
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Authors:
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N Arora; J Sandford; G F Browning; J R Sandy; P J Wright |
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Publication Detail:
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Type: Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't Date: 2006-04-18 |
Journal Detail:
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Title: Theriogenology Volume: 66 ISSN: 0093-691X ISO Abbreviation: Theriogenology Publication Date: 2006 Oct |
Date Detail:
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Created Date: 2006-09-18 Completed Date: 2006-11-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0421510 Medline TA: Theriogenology Country: United States |
Other Details:
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Languages: eng Pagination: 1530-6 Citation Subset: IM |
Affiliation:
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Department of Veterinary Science, The University of Melbourne, Werribee 3030, Vic., Australia. n.arora@pgrad.unimelb.edu.au |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cervix Uteri / physiology Colony Count, Microbial Contrast Media Dog Diseases / microbiology*, pathology* Dogs Endometrial Hyperplasia / microbiology, pathology, radiography, veterinary* Escherichia coli / growth & development* Escherichia coli Infections / microbiology, pathology, veterinary* Estrous Cycle / drug effects, physiology Female Ovariectomy / veterinary Progesterone / blood Uterine Diseases / microbiology, pathology*, veterinary* Vaginal Smears |
| Chemical | |
Reg. No./Substance:
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0/Contrast Media; 57-83-0/Progesterone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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