| A model for chronic quantitative studies of colorectal sensitivity using balloon distension in conscious mice -- effects of opioid receptor agonists. | |
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MedLine Citation:
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PMID: 12846725 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the current study, colorectal distension (CRD) was performed in conscious mice, in order to study visceral (colon) sensitivity. Electrodes were chronically implanted into the external oblique muscle to obtain the electromyographic (EMG) response to CRD. CRD was performed using a computerized system, which inflated the balloon with air to the desired pressures. An increasing (10-80 mmHg) and a repeated (12 x 55 mmHg) phasic paradigm with distensions lasting 10 s and with 5-min intervals were used. The EMG recordings were linearly correlated to intracolonic pressures between 10 and 80 mmHg, which are characteristic of the visceromotor response (VMR). Repeated phasic distensions at 55 mmHg resulted in a stable VMR in female mice, but an increasing VMR in male mice. Interestingly, the duration of the VMR was about 5 s, which is shorter than the actual duration of the distension. U-69593 and fentanyl (selective kappa and mu opioid receptor agonists) significantly reduced the VMR at subcutaneous doses of 0.5 and 0.05 mg x kg-1, respectively. In conclusion, a CRD model for repetitive quantitative studies of colorectal sensitivity and evaluation of pharmacological modulation of visceral sensitivity in conscious mice is presented. |
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Authors:
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M Larsson; S Arvidsson; C Ekman; A Bayati |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society Volume: 15 ISSN: 1350-1925 ISO Abbreviation: Neurogastroenterol. Motil. Publication Date: 2003 Aug |
Date Detail:
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Created Date: 2003-07-08 Completed Date: 2003-09-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9432572 Medline TA: Neurogastroenterol Motil Country: England |
Other Details:
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Languages: eng Pagination: 371-81 Citation Subset: IM |
Affiliation:
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Department of Integrative Pharmacology, Research Area CV & GI, Preclinical R&D, AstraZeneca, Mölndal, Sweden. marie.h.larsson@astrazenca.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Abdominal Muscles
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drug effects,
physiology Analgesics, Opioid / pharmacology Animals Benzeneacetamides* Colon / drug effects, physiology* Consciousness Electromyography Female Fentanyl / pharmacology Male Mice Models, Animal Muscle Contraction / drug effects Nociceptors / drug effects, physiology* Pain Measurement / methods* Physical Stimulation Pyrrolidines / pharmacology Receptors, Opioid / agonists* Rectum / drug effects, physiology* Sex Characteristics |
| Chemical | |
Reg. No./Substance:
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0/Analgesics, Opioid; 0/Benzeneacetamides; 0/Pyrrolidines; 0/Receptors, Opioid; 437-38-7/Fentanyl; 96744-75-1/U 69593 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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