Document Detail


A model for acute carbon monoxide poisoning in conscious rats.
MedLine Citation:
PMID:  8840478     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
These experiments were designed to establish an animal model of acute carbon monoxide (CO) poisoning in awake habituated rats. On the day before exposure, under a brief anesthesia, a Levine preparation (unilateral common carotid artery occlusion) was performed on group 1 (n = 8) and 2 (n = 28), but not on group 3 rats (n = 8). Group 1 rats were exposed to air as control. Groups 2 and 3 rats were exposed to 0.27% CO in air for 60 min [carboxyhemoglobin (HbCO) = 70%] followed by a 2-day recovery in air. The Levine preparation per se did not induce any detectable physiologic effects on group 1 rats. Identical cardiovascular and metabolic responses to CO occurred in groups 2 and 3. After the CO exposure, all group 3 rats lived for 2 days with normal neurologic index (NI). In group 2 (n = 25 post-CO), 84% of the rats showed increased NI and edema of the ipsilateral cerebral hemisphere, and 76% of the rats died 8.7 +/- 1.7 h after the CO exposure. NI correlated with the brain edema (rs = 0.748, P < 0.001) and inversely correlated with the survival time after the CO exposure (rs = -0.777, P < 0.001). We therefore may conclude that exposure of the Levine-prepared rats to 0.27% CO in air for 60 min will provide a valuable model for testing of different treatments for CO poisoning.
Authors:
J Jiang; I Tyssebotn
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Undersea & hyperbaric medicine : journal of the Undersea and Hyperbaric Medical Society, Inc     Volume:  23     ISSN:  1066-2936     ISO Abbreviation:  Undersea Hyperb Med     Publication Date:  1996 Jun 
Date Detail:
Created Date:  1996-10-31     Completed Date:  1996-10-31     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  9312954     Medline TA:  Undersea Hyperb Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  99-106     Citation Subset:  IM; S    
Affiliation:
Department of Physiology, University of Bergen, Norway.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Pressure / physiology
Bradycardia / physiopathology
Brain Edema
Carbon Dioxide / blood
Carbon Monoxide Poisoning* / blood,  physiopathology
Cause of Death
Disease Models, Animal*
Heart Rate / physiology
Male
Oxygen / blood
Rats
Rats, Wistar
Survival Rate
Chemical
Reg. No./Substance:
124-38-9/Carbon Dioxide; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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