Document Detail


The mode of action of heparin in plasma.
MedLine Citation:
PMID:  3238649     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The influence of heparin on prothrombin conversion in plasma was estimated by measuring prothrombin disappearance with the aid of staphylocoagulase or by calculation from the thrombin generation curve, compensating for simultaneous thrombin inactivation by plasma protease inhibitors. In thromboplastin-activated plasma the effect of heparin on prothrombinase (factor Xa-factor Va-phospholipid) is negligible compared to that on thrombin probably because both the extrinsic factor X activating complex and phospholipid bound factor Xa are insensitive to AT III-heparin action. In contact-activated plasma prothrombinase generation is inhibited by heparin, because heparin lowers the ambient concentrations of thrombin so that the feedback activation of factor VIII by thrombin is diminished. Consequently, the delayed factor Xa generation causes a lag phase in prothrombinase generation. We conclude that heparin in plasma acts by its thrombin scavenging properties. No direct action on prothrombinase or on the factor X activating enzyme needs to be assumed if one takes into account the effect of thrombin depletion on thrombin-mediated feedback reactions.
Authors:
S Béguin; T Lindhout; H C Hemker
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Thrombosis and haemostasis     Volume:  60     ISSN:  0340-6245     ISO Abbreviation:  Thromb. Haemost.     Publication Date:  1988 Dec 
Date Detail:
Created Date:  1989-05-01     Completed Date:  1989-05-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7608063     Medline TA:  Thromb Haemost     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  457-62     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of Limburg, Maastricht, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Heparin / blood,  pharmacology*
Humans
Kinetics
Prothrombin / metabolism
Thromboplastin / blood
Chemical
Reg. No./Substance:
9001-26-7/Prothrombin; 9005-49-6/Heparin; 9035-58-9/Thromboplastin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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