| A mixed pattern of endocrine cells in metaplastic Barrett's oesophagus. Evidence that the epithelium derives from a pluripotential stem cell. | |
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MedLine Citation:
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PMID: 2447038 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In order to characterize the differentiation of endocrine cells present in Barrett's oesophagus and to determine if they express a single or multiple hormonal pattern, endoscopic biopsies were taken from both the lesion and the fundus of 45 patients and studied at the light microscopical level. Conventional histology revealed three different epithelial patterns: gastric atrophic fundic, intestinal and junctional. A mixture of these patterns was present in 28 cases (62%) and the single type was identified in 17 cases (38%). The use of three silver staining methods and antibodies to human chromogranins allowed us to identify numerous endocrine cells in all but 1 case. Eleven sera against all the most common hormones stored in the endocrine cells of the gut were used to identify the main products of the cells. The following immunoreactivities were identified: 5-hydroxytryptamine (5-HT) (in 75% of the studied cases), somatostatin (87%), motilin (31%), pancreatic polypeptide (PP) (20%), glucose-dependent insulinotropic polypeptide (20%), gastrin (15%), glucagon (15%), peptide tyrosine tyrosine (13%), secretin (7%) and neurotensin (2%). No cholecystokinin-immunoreactive cells were identified. Our results indicated that, in Barrett's epithelium, both gastric and intestinal endocrine cells differentiate, in accordance with the variability of differentiation in the non-endocrine cells present in the different types of columnar epithelium. These findings provide support for the conclusion that Barrett's epithelium arises from a pluripotential stem cell capable of both gastric and intestinal differentiation. |
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Authors:
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G Rindi; A E Bishop; M J Daly; P Isaacs; F I Lee; J M Polak |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Histochemistry Volume: 87 ISSN: 0301-5564 ISO Abbreviation: Histochemistry Publication Date: 1987 |
Date Detail:
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Created Date: 1988-02-20 Completed Date: 1988-02-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0411300 Medline TA: Histochemistry Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 377-83 Citation Subset: IM |
Affiliation:
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Department of Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital, London, England. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aged Antibodies Barrett Esophagus / diagnosis, pathology* Endoscopy Epithelial Cells Esophageal Diseases / pathology* Esophagus / pathology Female Gastric Fundus / pathology Gastrointestinal Hormones / analysis* Humans Male Middle Aged Staining and Labeling Stomach / pathology |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Gastrointestinal Hormones |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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