| The mitochondrial outer membrane is not a major diffusion barrier for ADP in mouse heart skinned fibre bundles. | |
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MedLine Citation:
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PMID: 14722773 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The response of mitochondrial oxygen consumption to ADP in saponin-skinned cardiac fibre bundles has an apparent Km an order of magnitude higher than that in isolated mitochondria. Here we report that incubating skinned cardiac fibre bundles from wild-type mice or double-knockout mice lacking both cytosolic and mitochondrial creatine kinase (CK) with CK and creatine or with yeast hexokinase and glucose as extramitochondrial ADP-producing systems decreases the apparent Km of the bundles for ADP severalfold. We conclude that the affinity of mitochondria for ADP in mouse heart is of the same order of magnitude as that of isolated mitochondria, while the high apparent Km of the bundles is caused by diffusion gradients outside the mitochondria. |
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Authors:
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Olav Kongas; Marijke J Wagner; Frank ter Veld; Klaas Nicolay; Johannes H G M van Beek; Klaas Krab |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2004-01-14 |
Journal Detail:
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Title: Pflügers Archiv : European journal of physiology Volume: 447 ISSN: 0031-6768 ISO Abbreviation: Pflugers Arch. Publication Date: 2004 Mar |
Date Detail:
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Created Date: 2004-03-10 Completed Date: 2004-10-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0154720 Medline TA: Pflugers Arch Country: Germany |
Other Details:
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Languages: eng Pagination: 840-4 Citation Subset: IM |
Affiliation:
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Department of Mechanics and Applied Mathematics, Institute of Cybernetics, Tallinn Technical University, Akadeemia 21, 12618 Tallinn, Estonia. kongas@ioc.ee |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Diphosphate
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physiology* Animals Creatine Kinase / deficiency, genetics Creatine Kinase, Mitochondrial Form Diffusion Dose-Response Relationship, Drug Intracellular Membranes / enzymology, metabolism, physiology* Isoenzymes / deficiency, genetics Mice Mice, Inbred C57BL Mice, Knockout Mice, Transgenic Mitochondria, Heart / enzymology, genetics, metabolism* Myocardium / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Isoenzymes; 58-64-0/Adenosine Diphosphate; EC 2.7.3.2/Creatine Kinase; EC 2.7.3.2/Creatine Kinase, Mitochondrial Form |
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