Document Detail


A misexpression screen to identify regulators of Drosophila larval hemocyte development.
MedLine Citation:
PMID:  18757933     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In Drosophila, defense against foreign pathogens is mediated by an effective innate immune system, the cellular arm of which is composed of circulating hemocytes that engulf bacteria and encapsulate larger foreign particles. Three hemocyte types occur: plasmatocytes, crystal cells, and lamellocytes. The most abundant larval hemocyte type is the plasmatocyte, which is responsible for phagocytosis and is present either in circulation or in adherent sessile domains under the larval cuticle. The mechanisms controlling differentiation of plasmatocytes and their migration toward these sessile compartments are unclear. To address these questions we have conducted a misexpression screen using the plasmatocyte-expressed GAL4 driver Peroxidasin-GAL4 (Pxn-GAL4) and existing enhancer-promoter (EP) and EP yellow (EY) transposon libraries to systematically misexpress approximately 20% of Drosophila genes in larval hemocytes. The Pxn-GAL4 strain also contains a UAS-GFP reporter enabling hemocyte phenotypes to be visualized in the semitransparent larvae. Among 3412 insertions screened we uncovered 101 candidate hemocyte regulators. Some of these are known to control hemocyte development, but the majority either have no characterized function or are proteins of known function not previously implicated in hemocyte development. We have further analyzed three candidate genes for changes in hemocyte morphology, cell-cell adhesion properties, phagocytosis activity, and melanotic tumor formation.
Authors:
Martin Stofanko; So Yeon Kwon; Paul Badenhorst
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-30
Journal Detail:
Title:  Genetics     Volume:  180     ISSN:  0016-6731     ISO Abbreviation:  Genetics     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-09-16     Completed Date:  2008-11-17     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  253-67     Citation Subset:  IM    
Affiliation:
Institute of Biomedical Research, University of Birmingham, Edgbaston B15 2TT, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Animals
Crosses, Genetic
Drosophila Proteins / genetics
Drosophila melanogaster / embryology*,  genetics*
Female
Gene Expression Regulation, Developmental*
Genetic Techniques*
Hemocytes / cytology,  metabolism*
Larva / metabolism*
Male
Models, Genetic
Phagocytosis
Phenotype
Promoter Regions, Genetic
Signal Transduction
Grant Support
ID/Acronym/Agency:
//Medical Research Council
Chemical
Reg. No./Substance:
0/Drosophila Proteins
Comments/Corrections

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