Document Detail


A minimally invasive method for induction of myocardial infarction in an animal model using tungsten spirals.
MedLine Citation:
PMID:  19241139     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Most animal models use surgical thoracotomy with ligation of a coronary artery to induce myocardial infarction. Incision of the chest wall and myocardium affect remodeling after myocardial infarction. The aim of our study was to evaluate a new minimally invasive technique for inducing acute myocardial infarction in pigs. To this end, coronary angiography using a 6-F cardiac catheter was performed in 20 pigs. The cardiac catheter was advanced into the left circumflex artery (LCX) under electrocardiographic monitoring and small tungsten spirals were deployed in the vessel. LCX occlusion was verified by coronary angiography. Two days later, magnetic resonance imaging (MRI) was performed to estimate the extent of infarction. Thereafter, all animals were euthanized and the hearts stained with 2,3,5-triphenyltetrazolium chloride for histologic measurement of infarct size. Tungsten spirals were successfully placed in the LCX in all 20 pigs. About 13 of the 20 animals survived until the end of the experiment. The mean infarct size in the area supplied by the LCX was 4.4 +/- 2.3 cm(3) at MRI and 4.3 +/- 2.2 cm(3) at histology (r = 0.99, P < 0.001). No other myocardial regions showed infarction in any of the 13 pigs. Five of nine pigs requiring defibrillation due to ventricular fibrillation died because defibrillation was unsuccessful. One animal each died from pericarditis and pneumonia. Our results show that the minimally invasive method presented here enables reliable induction of myocardial infarction in a fairly straightforward manner. The 25% mortality rate associated with induction of myocardial infarction in our study is comparable to that reported by other investigators.
Authors:
Daniel Peukert; Michael Laule; Nicola Kaufels; Jörg Schnorr; Matthias Taupitz; Bernd Hamm; Marc Dewey
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Publication Detail:
Type:  Evaluation Studies; Journal Article     Date:  2009-02-25
Journal Detail:
Title:  The international journal of cardiovascular imaging     Volume:  25     ISSN:  1875-8312     ISO Abbreviation:  Int J Cardiovasc Imaging     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-05-18     Completed Date:  2009-07-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100969716     Medline TA:  Int J Cardiovasc Imaging     Country:  United States    
Other Details:
Languages:  eng     Pagination:  529-35     Citation Subset:  IM    
Affiliation:
Department of Radiology, Institut für Radiologie, Charité-Universitätsmedizin Berlin, Charitéplatz 1, Berlin, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Coronary Angiography
Disease Models, Animal*
Heart Catheterization
Magnetic Resonance Angiography
Myocardial Infarction / diagnosis*,  etiology*
Myocardium / pathology*
Reproducibility of Results
Swine
Swine, Miniature
Time Factors
Tungsten*
Chemical
Reg. No./Substance:
7440-33-7/Tungsten

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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