Document Detail

A miniaturized liquid core waveguide-capillary electrophoresis system with flow injection sample introduction and fluorometric detection using light-emitting diodes.
MedLine Citation:
PMID:  11575805     Owner:  NLM     Status:  MEDLINE    
A novel miniaturized capillary electrophoresis (CE) system is described where a Teflon AF-coated silica capillary serves both as the separation channel and as a transversely illuminated liquid core waveguide. This device uniquely uses flow injection (FI)-based split-flow sample introduction through a falling-drop interface. An H-channel structure fixed on a microscope glass slide utilizes a horizontal separation capillary with tubular sidearms on each end that serve as inlet and outlet flow-through electrode reservoirs. The inlet reservoir also functions as a falling-drop interface for coupling to the FI system. A blue LED is used as excitation source. A large-core optical fiber takes the emitted fluorescence to an inexpensive PMT with two layers of green plastic used for optical filtering. No focusing arrangement is needed. Continuous FI introduction of a series of 30-microL samples containing a mixture of of fluorescein isothiocyanate (FITC)-labeled amino acids allowed a throughput rate up to 144 samples/ h, with approximately 2% carryover and good precision (3.2% RSD). Baseline separation was achieved for FITC-labeled arginine, phenylalanine, glycine, and FITC in sodium tetraborate buffer (pH 9.5) with plate heights of 5.4-5.5 microm and plate numbers of 2.34 x 10(4)-2.37 x 10(4) under electrical field strengths of 214 V/cm for injection and 500 V/cm for separation (14-cm capillary, 48-microm i.d.). Detection limits (S/N = 3) were 1.3 microM for arginine and 1.9 microM for phenylalanine and glycine.
S L Wang; X J Huang; Z L Fang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical chemistry     Volume:  73     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  2001 Sep 
Date Detail:
Created Date:  2001-09-28     Completed Date:  2001-11-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4545-9     Citation Subset:  IM    
Research Center for Analytical Sciences, Northeastern University, Shenyang, PR China.
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MeSH Terms
Amino Acids / isolation & purification*
Arginine / isolation & purification
Electrophoresis, Capillary / methods*
Fluorometry / methods
Glycine / isolation & purification
Phenylalanine / isolation & purification
Reg. No./Substance:
0/Amino Acids; 56-40-6/Glycine; 63-91-2/Phenylalanine; 74-79-3/Arginine

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