Document Detail


mik1(+) G1-S transcription regulates mitotic entry in fission yeast.
MedLine Citation:
PMID:  11513869     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the fission yeast Schizosaccharomyces pombe Mik1p, in combination with Wee1p, is an important inhibitor of mitosis through direct phosphorylation of Cdc2p. Here we present the observation that mik1(+) is transcribed during G1- and S-phase in normally dividing cells. mik1(+) transcription is regulated by the MCB-DSC1 system, which controls expression of other genes at the G1-S interval. mik1(+) is shown to be an important target of MCB-DSC1 as it is epistatic for the mitotic delay phenotype displayed in cdc10-C4 cells, which are mutated in a component of DSC1. The mitotic delay in cdc10-C4 cells is bypassed by cdc2-1w, suggesting that mik1(+) acts directly on cdc2(+), with no checkpoint function involved. Thus, mik1(+) represents a new type of MCB-DSC1 regulated gene in fission yeast, whose gene product is exclusively expressed during G1- and S-phase to prevent premature mitosis during this cell cycle stage.
Authors:
S S Ng; M Anderson; S White; C J McInerny
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  503     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  2001 Aug 
Date Detail:
Created Date:  2001-08-21     Completed Date:  2001-09-20     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  131-4     Citation Subset:  IM    
Affiliation:
Division of Biochemistry and Molecular Biology, Institute of Biological and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
CDC2 Protein Kinase / metabolism
DNA Primers / genetics
DNA, Fungal / genetics,  metabolism
G1 Phase / genetics
Gene Expression Regulation, Fungal
Genes, Fungal*
Mitosis / genetics*
Molecular Sequence Data
Phenotype
Promoter Regions, Genetic
S Phase / genetics
Schizosaccharomyces / cytology*,  genetics*,  metabolism
Transcription, Genetic
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA, Fungal; EC 2.7.11.22/CDC2 Protein Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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