Document Detail

The migration of Langerhans' cells into and out of lymph nodes draining normal, carcinogen and antigen-treated sheep skin.
MedLine Citation:
PMID:  7512535     Owner:  NLM     Status:  MEDLINE    
Epidermal Langerhans' cell (LC) migration to the regional lymph node and beyond into central lymph was examined in sheep following topical application of the complete chemical carcinogen 7,12-dimethylbenz(a)anthracene (DMBA) or the contact sensitizing antigen 2,4,6-trinitrochlorobenzene (TNCB). This was facilitated by cannulating previously constructed pseudoafferent lymphatic vessels draining the skin treated with these agents or alternatively, the efferent lymphatic vessel of the regional lymph node. Application of DMBA resulted in a biphasic increase in LC migration. There was an initial increase in LC migration at 25 h with the maximum response (3.6 x 10(7) LC/h) occurring approximately 5 days after DMBA treatment. In contrast, the contact sensitizing antigen TNCB caused enhanced LC migration within minutes of the application of antigen (3.3 x 10(6) LC/h) and peak migration at 8-12 h. Examination of efferent lymph cells from the regional lymph node after DMBA treatment showed uncharacteristically large numbers of LC traversing the lymph node. These LC migration patterns suggest different mechanisms may trigger the migration of LC from skin after the application of DMBA to those associated with the normal processes of antigen presentation.
G W Dandie; F Y Watkins; S J Ragg; P E Holloway; H K Muller
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunology and cell biology     Volume:  72     ISSN:  0818-9641     ISO Abbreviation:  Immunol. Cell Biol.     Publication Date:  1994 Feb 
Date Detail:
Created Date:  1994-05-17     Completed Date:  1994-05-17     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8706300     Medline TA:  Immunol Cell Biol     Country:  AUSTRALIA    
Other Details:
Languages:  eng     Pagination:  79-86     Citation Subset:  IM    
Department of Pathology, University of Tasmania, Hobart, Australia.
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MeSH Terms
9,10-Dimethyl-1,2-benzanthracene / pharmacology
Antibodies, Monoclonal
Antigens, CD / immunology
Antigens, CD1
Cell Movement
Flow Cytometry
Fluorescent Antibody Technique
Langerhans Cells / physiology*
Lymph Nodes / cytology,  physiology*
Picryl Chloride / pharmacology
Skin / cytology,  drug effects*
Skin Physiological Phenomena
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, CD; 0/Antigens, CD1; 57-97-6/9,10-Dimethyl-1,2-benzanthracene; 88-88-0/Picryl Chloride

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