| A microwell array device capable of measuring single-cell oxygen consumption rates. | |
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MedLine Citation:
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PMID: 20084089 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Due to interest in cell population heterogeneity, the development of new technology and methodologies for studying single cells has dramatically increased in recent years. The ideal single cell measurement system would be high throughput for statistical relevance, would measure the most important cellular parameters, and minimize disruption of normal cell function. We have developed a microwell array device capable of measuring single cell oxygen consumption rates (OCR). This OCR device is able to diffusionally isolate single cells and enables the quantitative measurement of oxygen consumed by a single cell with fmol/min resolution in a non-invasive and relatively high throughput manner. A glass microwell array format containing fixed luminescent sensors allows for future incorporation of additional cellular parameter sensing capabilities. To demonstrate the utility of the OCR device, we determined the oxygen consumption rates of a small group of single cells (12 to 18) for three different cells lines: murine macrophage cell line RAW264.7, human epithelial lung cancer cell line A549, and human Barrett's esophagus cell line CP-D. |
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Authors:
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Timothy W Molter; Sarah C McQuaide; Martin T Suchorolski; Tim J Strovas; Lloyd W Burgess; Deirdre R Meldrum; Mary E Lidstrom |
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Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: Sensors and actuators. B, Chemical Volume: 135 ISSN: 0925-4005 ISO Abbreviation: - Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2010-1-19 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101149755 Medline TA: Sens Actuators B Chem Country: - |
Other Details:
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Languages: ENG Pagination: 678-686 Citation Subset: - |
Affiliation:
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University of Washington, Benjamin Hall Research Building, 616 NE Northlake Pl. Rm. 440, Seattle, WA 98105. |
Export Citation:
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Descriptor/Qualifier:
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| Grant Support | |
ID/Acronym/Agency:
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P50 HG002360-07//NHGRI NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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