Document Detail

The microtubule plus end-binding protein EB1 is involved in Sertoli cell plasticity in testicular seminiferous tubules.
MedLine Citation:
PMID:  17964570     Owner:  NLM     Status:  MEDLINE    
Sertoli cells of testis belong to a unique type of polarized epithelial cells and are essential for spermatogenesis. They form the blood-testis barrier at the base of seminiferous tubule. Their numerous long, microtubule-rich processes extend inward and associate with developing germ cells to sustain germ cell growth and differentiation. How Sertoli cells develop and maintain their elaborate processes has been an intriguing question. Here we showed that, by microinjecting lentiviral preparations into mouse testes of 29 days postpartum, we were able to specifically label individual Sertoli cells with GFP, thus achieving a clear view of their natural configurations together with associated germ cells in situ. Moreover, compared to other microtubule plus end-tracking proteins such as CLIP-170 and p150(Glued), EB1 was highly expressed in Sertoli cells and located along microtubule bundles in Sertoli cell processes. Stable overexpression of a GFP-tagged dominant-negative EB1 mutant disrupted microtubule organizations in cultured Sertoli cells. Furthermore, its overexpression in testis Sertoli cells altered their shapes. Sertoli cells in situ became rod-like, with decreased basal and lateral cell processes. Seminiferous tubule circularity and germ cell number were also reduced. These data indicate a requirement of proper microtubule arrays for Sertoli cell plasticity and function in testis.
Fubin Wang; Qiangge Zhang; Jingli Cao; Qiongping Huang; Xueliang Zhu
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-10-05
Journal Detail:
Title:  Experimental cell research     Volume:  314     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2007-12-06     Completed Date:  2008-02-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  213-26     Citation Subset:  IM    
Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, People's Republic of China.
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MeSH Terms
Cell Differentiation / physiology
Cell Polarity / physiology*
Cells, Cultured
Gene Expression Regulation, Developmental / genetics
Genetic Vectors
Germ Cells / cytology,  growth & development,  metabolism
Green Fluorescent Proteins / genetics
Mice, Inbred C57BL
Microtubule-Associated Proteins / genetics,  metabolism*
Microtubules / metabolism*,  ultrastructure
Protein Binding / physiology
Recombinant Fusion Proteins / genetics,  metabolism
Seminiferous Tubules / cytology,  growth & development,  metabolism*
Sertoli Cells / cytology,  metabolism*
Spermatogonia / cytology,  growth & development,  metabolism
Testis / cytology,  growth & development,  metabolism*
Reg. No./Substance:
0/EB1 microtubule binding proteins; 0/Microtubule-Associated Proteins; 0/Recombinant Fusion Proteins; 0/green fluorescent protein, Aequorea victoria; 147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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