Document Detail


A microsatellite marker within the duplicated D16S79 locus has a null allele; significance for linkage mapping.
MedLine Citation:
PMID:  8334897     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The dinucleotide repeat 16AC66f3 was characterised from D16S79A within a duplicated section of 16p13.11, which is duplicated on all normal chromosome 16's. This marker has a common null allele caused by polymorphism within one of the primer sites. A redesigned primer overcame this problem; however, this allowed amplification of two dinucleotide repeats, at D16S79A and D16S79B, with an overlapping and uninterpretable distribution of alleles. Thus, the 16AC66F3 marker with a null allele is potentially useful for linkage mapping, as it avoids the ambiguity associated with the genotyping of homologous AC repeats at this duplicated locus. The distribution of additional D16S79 RFLPs flanking FRA16A is clarified.
Authors:
H A Phillips; A D Thompson; H M Kozman; G R Sutherland; J C Mulley
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Cytogenetics and cell genetics     Volume:  64     ISSN:  0301-0171     ISO Abbreviation:  Cytogenet. Cell Genet.     Publication Date:  1993  
Date Detail:
Created Date:  1993-08-23     Completed Date:  1993-08-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0367735     Medline TA:  Cytogenet Cell Genet     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  131-2     Citation Subset:  IM    
Affiliation:
Department of Cytogenetics and Molecular Genetics, Adelaide Children's Hospital, North Adelaide, Australia.
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MeSH Terms
Descriptor/Qualifier:
Alleles
Base Sequence
Chromosome Mapping
Chromosomes, Human, Pair 16*
Genetic Markers*
Humans
Linkage (Genetics)
Molecular Sequence Data
Chemical
Reg. No./Substance:
0/Genetic Markers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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