| A microparticle enhanced nephelometric immunoassay (Nephelia) applied to thymulin measurement. | |
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MedLine Citation:
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PMID: 1806587 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This article describes a microparticle enhanced nephelometric immunoassay (Nephelia) applied to the quantification of the thymic peptide hormone thymulin. Nephelia uses antibody recognition by the antithymulin antiserum in a competitive reaction between free thymulin and thymulin bound to the microspheres. The binding between microsphere and thymulin is achieved with the aid of a protein carrier. The sensitivity of the competitive reaction varied with the protein carrier and the antiserum sample. The most efficient reaction was obtained with the thymulin-metallothionein-microsphere conjugates; as little as 5 pg/ml of thymulin could be detected. In adult human serum or in its ultra-filtrate, spiked synthetic thymulin was totally recovered. Measurement of thymulin in serum or ultra-filtrate samples demonstrated the presence of interference from molecules in the serum. Nephelia led to the same conclusions as those reported with other methods and performs as well, and is more simple to use than radio- or enzymo-immunoassays for thymulin measurement. |
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Authors:
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A Gartner; C Carles; P Montagne; M L Cuillière; J Duheille |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of immunoassay Volume: 12 ISSN: 0197-1522 ISO Abbreviation: J Immunoassay Publication Date: 1991 |
Date Detail:
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Created Date: 1992-05-08 Completed Date: 1992-05-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8007167 Medline TA: J Immunoassay Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 521-42 Citation Subset: IM |
Affiliation:
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Laboratoire de Nutrition Tropicale de l'ORSTOM, Montpellier, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Agglutination Tests Amino Acid Sequence Animals Antibodies / immunology, metabolism Binding, Competitive Child Humans Immunoassay / methods* Metallothionein Microspheres Molecular Sequence Data Nephelometry and Turbidimetry* Rabbits Reagent Kits, Diagnostic* Sensitivity and Specificity Thymic Factor, Circulating / analysis*, chemical synthesis, immunology Ultrafiltration |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Reagent Kits, Diagnostic; 78922-62-0/Thymic Factor, Circulating; 9038-94-2/Metallothionein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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