Document Detail


A micromethod for quantitative determination of iodoamino acids in thyroglobulin.
MedLine Citation:
PMID:  9135574     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe a new method for quantification of iodoamino acids after enzymatic hydrolysis of thyroglobulin. The procedure involves separation of monoiodotyrosine (MIT), di-iodotyrosine, tri-iodothyronine and thyroxine by reverse phase HPLC with a Vydac C18 stationary phase and a mobile phase of water-acetonitrile-acetic acid. The separation is monitored by sensitive spectrophotometric detection through a 96-well microplate system based on the catalytic Sandell-Kolthoff reaction of iodide on the oxidation of arsenic(III) by cerium(IV). This new microassay is particularly convenient because of its high sensitivity and its rapidity (less than 2 h). It can detect 1 pmol MIT and 0.5 pmol of the other three iodoamino acids with a recovery higher than 96%. Moreover, the 96-well microplate system allows many samples to be tested simultaneously and avoids the use of radiolabeled iodine.
Authors:
N Baudry; B Mallet; P J Lejeune; L Vinet; J L Franc
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of endocrinology     Volume:  153     ISSN:  0022-0795     ISO Abbreviation:  J. Endocrinol.     Publication Date:  1997 Apr 
Date Detail:
Created Date:  1997-05-19     Completed Date:  1997-05-19     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0375363     Medline TA:  J Endocrinol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  99-104     Citation Subset:  IM    
Affiliation:
Unité 38 INSERM, Faculté de Médicine, Marseille, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromatography, High Pressure Liquid
Diiodotyrosine / analysis
Monoiodotyrosine / analysis
Swine
Thyroglobulin / chemistry*
Thyroid Hormones / analysis*
Thyroxine / analysis
Triiodothyronine / analysis
Chemical
Reg. No./Substance:
0/Thyroid Hormones; 29592-76-5/Monoiodotyrosine; 66-02-4/Diiodotyrosine; 6893-02-3/Triiodothyronine; 7488-70-2/Thyroxine; 9010-34-8/Thyroglobulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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