| A micromethod for quantitative determination of iodoamino acids in thyroglobulin. | |
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MedLine Citation:
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PMID: 9135574 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We describe a new method for quantification of iodoamino acids after enzymatic hydrolysis of thyroglobulin. The procedure involves separation of monoiodotyrosine (MIT), di-iodotyrosine, tri-iodothyronine and thyroxine by reverse phase HPLC with a Vydac C18 stationary phase and a mobile phase of water-acetonitrile-acetic acid. The separation is monitored by sensitive spectrophotometric detection through a 96-well microplate system based on the catalytic Sandell-Kolthoff reaction of iodide on the oxidation of arsenic(III) by cerium(IV). This new microassay is particularly convenient because of its high sensitivity and its rapidity (less than 2 h). It can detect 1 pmol MIT and 0.5 pmol of the other three iodoamino acids with a recovery higher than 96%. Moreover, the 96-well microplate system allows many samples to be tested simultaneously and avoids the use of radiolabeled iodine. |
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Authors:
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N Baudry; B Mallet; P J Lejeune; L Vinet; J L Franc |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of endocrinology Volume: 153 ISSN: 0022-0795 ISO Abbreviation: J. Endocrinol. Publication Date: 1997 Apr |
Date Detail:
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Created Date: 1997-05-19 Completed Date: 1997-05-19 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0375363 Medline TA: J Endocrinol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 99-104 Citation Subset: IM |
Affiliation:
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Unité 38 INSERM, Faculté de Médicine, Marseille, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chromatography, High Pressure Liquid Diiodotyrosine / analysis Monoiodotyrosine / analysis Swine Thyroglobulin / chemistry* Thyroid Hormones / analysis* Thyroxine / analysis Triiodothyronine / analysis |
| Chemical | |
Reg. No./Substance:
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0/Thyroid Hormones; 29592-76-5/Monoiodotyrosine; 66-02-4/Diiodotyrosine; 6893-02-3/Triiodothyronine; 7488-70-2/Thyroxine; 9010-34-8/Thyroglobulin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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