Document Detail

A micromethod for chromosome preparation from individual hematopoietic colonies cultured in methylcellulose.
MedLine Citation:
PMID:  3891885     Owner:  NLM     Status:  MEDLINE    
We report a micromethod for chromosome preparation from individual hematopoietic colonies cultured in methylcellulose. The entire process was carried out on poly-Lysine (PL)-coated slides. Individual colonies were transferred into 10 microliter of 0.075 M KCl and placed on PL-coated slides. After hypotonic treatment of the colony cells and their attachment to the slides, the cells were fixed by a three-step procedure as follows: addition of a 30% fixative (3:1 methanol:acetic acid) diluted with the hypotonic solution, addition of 20% ethanol, and subsequent immersion of the slides in a 100% fixative. The slides were flame dried and Giemsa stained. Q- and G-banding techniques also were used. These procedures provided analyzable chromosome preparations, even from colonies containing fewer than 50 cells.
T Amenomori; M Tomonaga; T Matsuo; Y Yoshida; K Kuriyama; N Sadamori; M Ichimaru
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of cell cloning     Volume:  3     ISSN:  0737-1454     ISO Abbreviation:  Int. J. Cell Cloning     Publication Date:  1985 May 
Date Detail:
Created Date:  1985-07-31     Completed Date:  1985-07-31     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8308172     Medline TA:  Int J Cell Cloning     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  133-42     Citation Subset:  IM    
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MeSH Terms
Bone Marrow
Cell Separation
Cells, Cultured
Chromosome Banding
Cytological Techniques
Hematopoietic Stem Cells / ultrastructure*
Leukemia, Myeloid, Acute / genetics
Reg. No./Substance:

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