Document Detail


A microfluidic microelectrode array for simultaneous electrophysiology, chemical stimulation, and imaging of brain slices.
MedLine Citation:
PMID:  23042571     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to understand information processing in neural circuits, it is necessary to detect both electrical and chemical signaling with high spatial and temporal resolution. Although the primary currency of neural information processing is electrical, many of the downstream effects of the electrical signals on the circuits that generate them are dependent on activity-dependent increases in intracellular calcium concentration. It is therefore of great utility to be able to record electrical signals in neural circuits at multiple sites, while at the same time detecting optical signals from reporters of intracellular calcium levels. We describe here a microfluidic multi-electrode array (MMEA) capable of high-resolution extracellular recording from brain slices that is optically compatible with calcium imaging at single cell resolution. We show the application of the MMEA device to record waves of spontaneous activity in developing cortical slices and to perform multi-site extracellular recordings during simultaneous calcium imaging of activity. The MMEA has the unique capability to simultaneously allow focal electrical and chemical stimuli at different locations of the surface of a brain slice.
Authors:
Adina Scott; Keiko Weir; Curtis Easton; Wilson Huynh; William J Moody; Albert Folch
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Lab on a chip     Volume:  13     ISSN:  1473-0189     ISO Abbreviation:  Lab Chip     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-24     Completed Date:  2013-10-30     Revised Date:  2014-02-23    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  527-35     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / cytology,  drug effects,  physiology*
Calcium Signaling
Electrophysiology* / instrumentation
Female
Mice
Microelectrodes
Microfluidic Analytical Techniques* / instrumentation
Stimulation, Chemical
Grant Support
ID/Acronym/Agency:
R01 EB007526/EB/NIBIB NIH HHS; R01 EB007526-01/EB/NIBIB NIH HHS
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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