Document Detail


A microfluidic device to investigate axon targeting by limited numbers of purified cortical projection neuron subtypes.
MedLine Citation:
PMID:  23034677     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
While much is known about general controls over axon guidance of broad classes of projection neurons (those with long-distance axonal connections), molecular controls over specific axon targeting by distinct neuron subtypes are poorly understood. Corticospinal motor neurons (CSMN) are prototypical and clinically important cerebral cortex projection neurons; they are the brain neurons that degenerate in amyotrophic lateral sclerosis (ALS) and related motor neuron diseases, and their injury is central to the loss of motor function in spinal cord injury. Primary culture of purified immature murine CSMN has been recently established, using either fluorescence-activated cell sorting (FACS) or immunopanning, enabling a previously unattainable level of subtype-specific investigation, but the resulting number of CSMN is quite limiting for standard approaches to study axon guidance. We developed a microfluidic system specifically designed to investigate axon targeting of limited numbers of purified CSMN and other projection neurons in culture. The system contains two chambers for culturing target tissue explants, allowing for biologically revealing axonal growth "choice" experiments. This device will be uniquely enabling for investigation of controls over axon growth and neuronal survival of many types of neurons, particularly those available only in limited numbers.
Authors:
Suzanne Tharin; Chandrasekhar R Kothapalli; Pembe Hande Ozdinler; Lincoln Pasquina; Seok Chung; Johanna Varner; Sarra DeValence; Roger Kamm; Jeffrey D Macklis
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Integrative biology : quantitative biosciences from nano to macro     Volume:  4     ISSN:  1757-9708     ISO Abbreviation:  Integr Biol (Camb)     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-23     Completed Date:  2013-04-05     Revised Date:  2013-11-05    
Medline Journal Info:
Nlm Unique ID:  101478378     Medline TA:  Integr Biol (Camb)     Country:  England    
Other Details:
Languages:  eng     Pagination:  1398-405     Citation Subset:  IM    
Affiliation:
Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Axons / physiology*,  ultrastructure
Cell Survival
Cells, Cultured
Cellular Microenvironment
Cerebral Cortex / cytology
Coculture Techniques
Equipment Design
Mice
Microfluidic Analytical Techniques*
Motor Neurons / classification,  physiology*,  ultrastructure
Spinal Cord / cytology
Grant Support
ID/Acronym/Agency:
NS41590/NS/NINDS NIH HHS; NS45523/NS/NINDS NIH HHS; NS49553/NS/NINDS NIH HHS; R01 NS041590/NS/NINDS NIH HHS; R01 NS045523/NS/NINDS NIH HHS
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