Document Detail


A microfluidic chip for real-time studies of the volume of single cells.
MedLine Citation:
PMID:  19107281     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report a microfluidic chip that is capable of measuring volume changes in single cells in real-time. Single eukaryotic cells were immobilized in the sensing area and changes in volume in response to hypotonic challenges and drugs were measured using the electrical impedance method. Experiments on MDCK cells showed that the maximum swelling and the time course of swelling vary between individual cells following hypotonic stimulation. The microfluidic chip allows, rapid and convenient change of solutions, enabling detailed studies of various drugs and chemicals that may play important role in cell physiology at the single cell level.
Authors:
Susan Z Hua; Thomas Pennell
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2008-10-23
Journal Detail:
Title:  Lab on a chip     Volume:  9     ISSN:  1473-0197     ISO Abbreviation:  Lab Chip     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-24     Completed Date:  2009-03-24     Revised Date:  2014-09-08    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  251-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cell Size* / drug effects
Dogs
Epithelial Cells / physiology*
Hypotonic Solutions / pharmacology
Microfluidic Analytical Techniques / methods*
Time Factors
Grant Support
ID/Acronym/Agency:
DK77302/DK/NIDDK NIH HHS; K25 DK077302/DK/NIDDK NIH HHS; K25 DK077302-02/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Hypotonic Solutions
Comments/Corrections

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