Document Detail


A microfluidic bioreactor based on hydrogel-entrapped E. coli: cell viability, lysis, and intracellular enzyme reactions.
MedLine Citation:
PMID:  12530814     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Viable E. coli cells were entrapped in hydrogel micropatches photopolymerized within microfluidic systems. The microfluidic channels and the micropatches have sizes on the order of 100-500 microm. Small molecules, such as dyes and surfactants, present in the solution surrounding the hydrogel, are able to diffuse into the gel and encounter the cells, but the cells are sufficiently large to be retained. For example, sodium dodecyl sulfate is a lysis agent that is able to penetrate the hydrogel and disrupt the cellular membrane. Entrapment of viable cells within hydrogels, followed by lysis, could provide a convenient means for preparing biocatalysts without the need for enzyme extraction and purification. Hydrogel-immobilized cells are able to carry out chemical reactions within microfluidic channels. Specifically, a nonfluorescent dye, BCECF-AM, is able to penetrate both the hydrogel and the bacterial membrane and be converted into a fluorescent form (BCECF) by the interior cellular machinery. These results suggest that cells immobilized within microfluidic channels can act as sensors for small molecules and as bioreactors for carrying out reactions.
Authors:
Jinseok Heo; K Joseph Thomas; Gi Hun Seong; Richard M Crooks
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Analytical chemistry     Volume:  75     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  2003 Jan 
Date Detail:
Created Date:  2003-01-17     Completed Date:  2003-04-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  22-6     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Texas A&M University, P.O. Box 30012, College Station, Texas 77842-3012, USA.
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MeSH Terms
Descriptor/Qualifier:
Bioreactors / microbiology*
Cell Survival
Cells, Immobilized / cytology,  enzymology,  metabolism
Escherichia coli / cytology*,  enzymology*,  metabolism
Fluoresceins / chemistry
Fluorescence
Hydrogels*
Microchemistry*
Molecular Structure
Chemical
Reg. No./Substance:
0/Fluoresceins; 0/Hydrogels; 85138-49-4/2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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