Document Detail


A microfluidic approach for anticancer drug analysis based on hydrogel encapsulated tumor cells.
MedLine Citation:
PMID:  20381684     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A novel method based on fluorescence detection of hydrogel encapsulated cells in microchannels was developed for anticancer drug analysis. In this work, human hepatoma HepG2 cells and human lung epithelial A549 cells were simultaneously immobilized inside two different shapes of three-dimensional hydrogel microstructures using photolithography approach on a same array. Microarrays of living cells offer the potential for parallel detection of many cells and thereby enable high-throughput assays. Using a photolithographic setup, we investigated the prepolymer composition and crosslinking parameters that influenced cell viability inside photocrosslinked hydrogels. The viability of cells encapsulated inside hydrogel microstructures was higher than 90% under optimized photocrosslinking conditions. The cells were further cultured under stable conditions and remained viable for at least three days that were able to carry out cell-based assays. Furthermore, we studied the variation of two intracellular redox parameters (glutathione and reactive oxygen species) in anticancer drug-induced apoptosis in HepG2 and A549 cells. Two anticancer drugs exhibited distinct effects on the levels of intracellular glutathione and reactive oxygen species, indicating the selectivity of these drugs on the disturbance of redox balance within cells. The established platform provides a convenient and fast method for monitoring the effect of anticancer drugs on tumor cells, which is very useful for fundamental biomedical research.
Authors:
Dan Gao; Jiangjiang Liu; Hui-Bin Wei; Hai-Fang Li; Guang-Sheng Guo; Jin-Ming Lin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-12
Journal Detail:
Title:  Analytica chimica acta     Volume:  665     ISSN:  1873-4324     ISO Abbreviation:  Anal. Chim. Acta     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-12     Completed Date:  2010-06-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370534     Medline TA:  Anal Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  7-14     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier B.V. All rights reserved.
Affiliation:
School of Science, Beijing University of Chemical Technology, Beijing 100029, China.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents / chemistry*
Cell Line, Tumor
Glutathione / metabolism
Hep G2 Cells
Humans
Hydrogels / chemistry*
Microfluidic Analytical Techniques / methods*
Polymers / chemistry
Reactive Oxygen Species / metabolism
Tissue Array Analysis
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Hydrogels; 0/Polymers; 0/Reactive Oxygen Species; 70-18-8/Glutathione

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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