Document Detail

A microfabricated fluorescence-activated cell sorter.
MedLine Citation:
PMID:  10545919     Owner:  NLM     Status:  MEDLINE    
We have demonstrated a disposable microfabricated fluorescence-activated cell sorter (microFACS) for sorting various biological entities. Compared with conventional FACS machines, the microFACS provides higher sensitivity, no cross-contamination, and lower cost. We have used microFACS chips to obtain substantial enrichment of micron-sized fluorescent bead populations of differing colors. Furthermore, we have separated Escherichia coli cells expressing green fluorescent protein from a background of nonfluorescent E. coli cells and shown that the bacteria are viable after extraction from the sorting device. These sorters can function as stand-alone devices or as components of an integrated microanalytical chip.
A Y Fu; C Spence; A Scherer; F H Arnold; S R Quake
Related Documents :
10525679 - Green fluorescent protein as a cell-labeling tool and a reporter of gene expression in ...
16233529 - High-throughput screening for isolation of enhanced green fluorescent protein expressin...
15164239 - Recovery of gfp-labeled bacteria for culturing and molecular analysis after cell sortin...
18953349 - A noncytotoxic dsred variant for whole-cell labeling.
19949979 - Development of a three-dimensional in vitro model for longitudinal observation of cell ...
17680759 - Genetically encoded intracellular sensors based on fluorescent proteins.
16099639 - Molecular control of cell polarity and asymmetric cell division in drosophila neuroblasts.
22358759 - Suppressive effect of spinach extract on the formation of melanin in b16 mouse melanoma...
18228349 - Generation of continuously growing b cell lines by epstein-barr virus transformation.
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Nature biotechnology     Volume:  17     ISSN:  1087-0156     ISO Abbreviation:  Nat. Biotechnol.     Publication Date:  1999 Nov 
Date Detail:
Created Date:  1999-12-15     Completed Date:  1999-12-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9604648     Medline TA:  Nat Biotechnol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1109-11     Citation Subset:  IM    
Department of Applied Physics, California Institute of Technology, Pasadena, CA 91125, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Escherichia coli / cytology
Flow Cytometry / instrumentation*,  methods*
Green Fluorescent Proteins
Luminescent Proteins / metabolism
Reg. No./Substance:
0/Luminescent Proteins; 0/Silicones; 147336-22-9/Green Fluorescent Proteins
Comment In:
Nat Biotechnol. 1999 Nov;17(11):1061-2   [PMID:  10545906 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Micropatterned immobilization of a G protein-coupled receptor and direct detection of G protein acti...
Next Document:  Purification of recombinant proteins by fusion with thermally-responsive polypeptides.