Document Detail


A microfabricated fluorescence-activated cell sorter.
MedLine Citation:
PMID:  10545919     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have demonstrated a disposable microfabricated fluorescence-activated cell sorter (microFACS) for sorting various biological entities. Compared with conventional FACS machines, the microFACS provides higher sensitivity, no cross-contamination, and lower cost. We have used microFACS chips to obtain substantial enrichment of micron-sized fluorescent bead populations of differing colors. Furthermore, we have separated Escherichia coli cells expressing green fluorescent protein from a background of nonfluorescent E. coli cells and shown that the bacteria are viable after extraction from the sorting device. These sorters can function as stand-alone devices or as components of an integrated microanalytical chip.
Authors:
A Y Fu; C Spence; A Scherer; F H Arnold; S R Quake
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Nature biotechnology     Volume:  17     ISSN:  1087-0156     ISO Abbreviation:  Nat. Biotechnol.     Publication Date:  1999 Nov 
Date Detail:
Created Date:  1999-12-15     Completed Date:  1999-12-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9604648     Medline TA:  Nat Biotechnol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1109-11     Citation Subset:  IM    
Affiliation:
Department of Applied Physics, California Institute of Technology, Pasadena, CA 91125, USA.
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MeSH Terms
Descriptor/Qualifier:
Escherichia coli / cytology
Flow Cytometry / instrumentation*,  methods*
Green Fluorescent Proteins
Luminescent Proteins / metabolism
Silicones
Chemical
Reg. No./Substance:
0/Luminescent Proteins; 0/Silicones; 147336-22-9/Green Fluorescent Proteins
Comments/Corrections
Comment In:
Nat Biotechnol. 1999 Nov;17(11):1061-2   [PMID:  10545906 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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