Document Detail


A microbiota signature associated with experimental food allergy promotes allergic sensitization and anaphylaxis.
MedLine Citation:
PMID:  23201093     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Commensal microbiota play a critical role in maintaining oral tolerance. The effect of food allergy on the gut microbial ecology remains unknown.
OBJECTIVE: We sought to establish the composition of the gut microbiota in experimental food allergy and its role in disease pathogenesis.
METHODS: Food allergy-prone mice with a gain-of-function mutation in the IL-4 receptor α chain (Il4raF709) and wild-type (WT) control animals were subjected to oral sensitization with chicken egg ovalbumin (OVA). Enforced tolerance was achieved by using allergen-specific regulatory T (Treg) cells. Community structure analysis of gut microbiota was performed by using a high-density 16S rDNA oligonucleotide microarrays (PhyloChip) and massively parallel pyrosequencing of 16S rDNA amplicons.
RESULTS: OVA-sensitized Il4raF709 mice exhibited a specific microbiota signature characterized by coordinate changes in the abundance of taxa of several bacterial families, including the Lachnospiraceae, Lactobacillaceae, Rikenellaceae, and Porphyromonadaceae. This signature was not shared by similarly sensitized WT mice, which did not exhibit an OVA-induced allergic response. Treatment of OVA-sensitized Il4raF709 mice with OVA-specific Treg cells led to a distinct tolerance-associated signature coincident with the suppression of the allergic response. The microbiota of allergen-sensitized Il4raF709 mice differentially promoted OVA-specific IgE responses and anaphylaxis when reconstituted in WT germ-free mice.
CONCLUSION: Mice with food allergy exhibit a specific gut microbiota signature capable of transmitting disease susceptibility and subject to reprogramming by enforced tolerance. Disease-associated microbiota may thus play a pathogenic role in food allergy.
Authors:
Magali Noval Rivas; Oliver T Burton; Petra Wise; Yu-qian Zhang; Suejy A Hobson; Maria Garcia Lloret; Christel Chehoud; Justin Kuczynski; Todd DeSantis; Janet Warrington; Embriette R Hyde; Joseph F Petrosino; Georg K Gerber; Lynn Bry; Hans C Oettgen; Sarkis K Mazmanian; Talal A Chatila
Related Documents :
25226823 - Development of the helius food frequency questionnaires: ethnic-specific questionnaires...
1822943 - The current status of food-borne parasitic zoonoses in indonesia.
1172863 - Controlled study of the cytotoxic food test.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-11-30
Journal Detail:
Title:  The Journal of allergy and clinical immunology     Volume:  131     ISSN:  1097-6825     ISO Abbreviation:  J. Allergy Clin. Immunol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-12-25     Completed Date:  2013-03-06     Revised Date:  2014-04-10    
Medline Journal Info:
Nlm Unique ID:  1275002     Medline TA:  J Allergy Clin Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  201-12     Citation Subset:  AIM; IM    
Copyright Information:
Copyright © 2012 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc. All rights reserved.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Administration, Oral
Allergens / administration & dosage,  immunology
Anaphylaxis / immunology,  microbiology
Animals
Disease Susceptibility / immunology
Female
Food / adverse effects
Food Hypersensitivity / immunology*,  microbiology*,  therapy
Food Microbiology*
Immune Tolerance / immunology
Immunotherapy, Adoptive
Intestinal Mucosa / immunology,  microbiology
Male
Metagenome / genetics,  immunology*
Mice
Mice, Transgenic
Phylogeny
T-Lymphocytes, Regulatory / cytology,  immunology
Grant Support
ID/Acronym/Agency:
AI 080002/AI/NIAID NIH HHS; AI007512/AI/NIAID NIH HHS; AI100889/AI/NIAID NIH HHS; DK034854/DK/NIDDK NIH HHS; DK078938/DK/NIDDK NIH HHS; HL07627/HL/NHLBI NIH HHS; R01 AI085090/AI/NIAID NIH HHS; R21 AI080002/AI/NIAID NIH HHS; T32 AI007512/AI/NIAID NIH HHS; T32 GM008231/GM/NIGMS NIH HHS; T32 HL007627/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Allergens
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Evidences for the regulation of GnRH and GTH expression by GnIH in the goldfish, Carassius auratus.
Next Document:  The effect of Neuroform stent-assisted coil embolization of wide-necked intracranial aneurysms and c...