Document Detail

A microbial biosensor based on bacterial cells immobilized on chitosan matrix.
MedLine Citation:
PMID:  19196553     Owner:  NLM     Status:  MEDLINE    
A bio-electrochemical system consisting of Gluconobacter oxydans DSM 2343 cells as a biological material and carbon nanotube (CNT)-free and CNT-modified chitosan as immobilizing matrices has been developed. The measurement was based on the respiratory activity of the cells estimated by the oxygen consumption at -0.7 V (versus the Ag|AgCl reference electrode) due to the metabolic activity in the presence of substrates. The system was calibrated and dependence of signal amplitude on the measuring conditions and cell amount was studied as well as the substrate specificity, pH, temperature and working potential. The biosensors (CNT-modified and unmodified) were demonstrated for the quantification of glucose in the range of 0.05-1.0 mM, at 30 degrees C and pH 7.0 with the 40 s of response time. The linear relationships between sensor response (y; microA/cm(2)) and substrate concentration (x; mM) were defined by the equations of y=1.160x+0.151 (R(2)=0.990) and y=1.261x+0.197 (R(2)=0.982), respectively. All other data were also given as comparison of two systems one with CNT-modified and CNT-free.
Dilek Odaci; Suna Timur; Azmi Telefoncu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-01-16
Journal Detail:
Title:  Bioelectrochemistry (Amsterdam, Netherlands)     Volume:  75     ISSN:  1878-562X     ISO Abbreviation:  Bioelectrochemistry     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-03-16     Completed Date:  2009-06-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100953583     Medline TA:  Bioelectrochemistry     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  77-82     Citation Subset:  IM    
Ege University, Faculty of Science, Biochemistry Department, 35100 Bornova-Izmir, Turkey.
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MeSH Terms
Biosensing Techniques / methods*
Cells, Immobilized
Chitosan / chemistry*
Gluconobacter oxydans / metabolism*
Hydrogen-Ion Concentration
Microscopy, Electron, Scanning
Nanotubes, Carbon / chemistry,  ultrastructure
Substrate Specificity
Reg. No./Substance:
0/Nanotubes, Carbon; 50-99-7/Glucose; 9012-76-4/Chitosan

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