| microRNA miR-275 is indispensable for blood digestion and egg development in the mosquito Aedes aegypti. | |
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MedLine Citation:
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PMID: 21115818 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mosquito Aedes aegypti is the major vector of arboviral diseases, particularly of Dengue fever, of which there are more than 100 million cases annually. Mosquitoes, such as A. aegypti, serve as vectors for disease pathogens because they require vertebrate blood for their egg production. Pathogen transmission is tightly linked to repeated cycles of obligatory blood feeding and egg maturation. Thus, the understanding of mechanisms governing egg production is necessary to develop approaches that limit the spread of mosquito-borne diseases. Previous studies have identified critical roles of hormonal- and nutrition-based target of rapamycin (TOR) pathways in controlling blood-meal-mediated egg maturation in mosquitoes. In this work, we uncovered another essential regulator of blood-meal-activated processes, the microRNA miR-275. The depletion of this microRNA in A. aegypti females after injection of its specific antagomir resulted in severe defects in blood digestion, fluid excretion, and egg development, clearly demonstrating that miR-275 is indispensable for these physiological processes. miR-275 exhibits an expression profile that suggests its regulation by a steroid hormone, 20-hydroxyecdysone (20E). In vitro organ culture experiments demonstrated that miR-275 is induced by this hormone in the presence of amino acids, indicative of a dual regulation by 20E and TOR. This report has uncovered the critical importance of microRNAs in controlling blood-meal-activated physiological events required for completion of egg development in mosquito disease vectors. |
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Authors:
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Bart Bryant; Warren Macdonald; Alexander S Raikhel |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-11-29 |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 107 ISSN: 1091-6490 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-12-29 Completed Date: 2011-03-01 Revised Date: 2011-08-01 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
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Languages: eng Pagination: 22391-8 Citation Subset: IM |
Affiliation:
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Department of Entomology, Institute for Integrative Genome Biology, University of California, Riverside, CA 92521, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aedes
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genetics*,
metabolism,
physiology Amino Acid Sequence Animals Blood / metabolism Blotting, Western Ecdysterone / pharmacology Fat Body / metabolism* Feeding Behavior Female Gene Expression Profiling Gene Expression Regulation / drug effects Insect Proteins / genetics, metabolism MicroRNAs / genetics*, metabolism Molecular Sequence Data Oocytes / growth & development, metabolism* RNA Interference Rats Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Signal Transduction TOR Serine-Threonine Kinases / genetics, metabolism Tissue Culture Techniques |
| Grant Support | |
ID/Acronym/Agency:
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R37 AI024716-24/AI/NIAID NIH HHS; R37 AI244716/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Insect Proteins; 0/MicroRNAs; 0/microRNA miR-275, Aedes aegypti; 5289-74-7/Ecdysterone; EC 2.7.1.1/TOR Serine-Threonine Kinases |
| Comments/Corrections | |
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