Document Detail


microRNA in the ovine mammary gland during early pregnancy: spatial and temporal expression of miR-21, miR-205 and miR-200.
MedLine Citation:
PMID:  23269700     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The mammary gland undergoes extensive remodelling between the beginning of pregnancy and lactation; this involves cellular processes including cell proliferation, differentiation and apoptosis, all of which are under the control of numerous regulators. To unravel the role played by miRNA, we describe here 47 new ovine miRNA cloned from mammary gland in early pregnancy displaying strong similarities with those already identified in the cow, human or mouse. A microarray study of miRNA variations in the adult ovine mammary gland during pregnancy and lactation showed that one hundred miRNA are regulated according to three principal patterns of expression: a decrease in early pregnancy, a peak at mid-pregnancy or an increase throughout late pregnancy and lactation. One miRNA displaying each pattern (miR-21, miR-205 and miR-200b) was analyzed by qRT-PCR. Variations in expression were confirmed for all three miRNA. Using in situ hybridization, both miR-21 and miR-200 were detected in luminal mammary epithelial cells when expressed, whereas miR-205 was expressed in basal cells during the first half of pregnancy and then in luminal cells during the second half. We therefore conclude that miR-21 is strongly expressed in the luminal cells of the normal mammary gland during early pregnancy when extensive cell proliferation occurs. In addition, we show that miR-205 and miR-200 are co-expressed in luminal cells, but only during the second half of pregnancy. These two miRNA may cooperate in order to maintain epithelial status by repressing an EMT-like programme, in order to achieve and preserve the secretory phenotype of mammary epithelial cells.
Authors:
Laurent Galio; Stéphanie Droineau; Patrick Yeboah; Hania Boudiaf; Stephan Bouet; Sandrine Truchet; Eve Devinoy
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-26
Journal Detail:
Title:  Physiological genomics     Volume:  -     ISSN:  1531-2267     ISO Abbreviation:  Physiol. Genomics     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9815683     Medline TA:  Physiol Genomics     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Institut National de la Recherche Agronomique.
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