Document Detail


The microRNA expression changes associated with malignancy and SDHB mutation in pheochromocytoma.
MedLine Citation:
PMID:  22241719     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Currently, the diagnosis of malignant pheochromocytoma can only be made when there is clinical evidence of metastasis or extensive local invasion. Thus, there is a need for new diagnostic marker(s) to identify tumors with malignant potential. The purpose of this study was to identify microRNAs (miRNAs) that are differentially expressed between benign and malignant pheochromocytomas and assess their diagnostic accuracy. Toward this aim, we analyzed miRNA expression in benign and malignant pheochromocytoma tumor samples using whole genome microarray profiling. Microarray analysis identified eight miRNAs that were significantly differentially expressed between benign and malignant pheochromocytomas. We measured a subset of these miRNAs directly by RT-PCR and found that miR-483-5p, miR-183, and miR-101 had significantly higher expression in malignant tumors as compared to their benign counterparts. Area under the receiver operating curve (AUC) analysis indicated that miR-483-5p, miR-101, and miR-183 could be useful diagnostic markers for distinguishing malignant from benign pheochromocytomas. In addition, these miRNAs could be detected in pheochromocytoma patient serum. Overall our data suggest that misexpression of miR-483-5p, miR-101, and miR-183 is associated with malignant pheochromocytoma.
Authors:
E Patterson; R Webb; A Weisbrod; B Bian; M He; L Zhang; A K Holloway; R Krishna; N Nilubol; K Pacak; E Kebebew
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural     Date:  2012-04-10
Journal Detail:
Title:  Endocrine-related cancer     Volume:  19     ISSN:  1479-6821     ISO Abbreviation:  Endocr. Relat. Cancer     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-04-12     Completed Date:  2012-10-17     Revised Date:  2012-11-30    
Medline Journal Info:
Nlm Unique ID:  9436481     Medline TA:  Endocr Relat Cancer     Country:  England    
Other Details:
Languages:  eng     Pagination:  157-66     Citation Subset:  IM    
Affiliation:
Endocrine Oncology Section, NIH/NCI/Surgery Branch, National Cancer Institute, NIH, Hatfield Clinical Research Center, Bethesda, Maryland 20892, USA.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adrenal Gland Neoplasms / enzymology,  genetics*,  metabolism
Adult
Aged
Female
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Neoplastic
Germ-Line Mutation*
Humans
Male
MicroRNAs / biosynthesis,  blood,  genetics*
Middle Aged
Oligonucleotide Array Sequence Analysis / methods
Pheochromocytoma / enzymology,  genetics*,  metabolism
RNA, Neoplasm / chemistry,  genetics
Real-Time Polymerase Chain Reaction
Succinate Dehydrogenase / genetics*
Tumor Markers, Biological / biosynthesis,  blood,  genetics
Young Adult
Chemical
Reg. No./Substance:
0/MicroRNAs; 0/RNA, Neoplasm; 0/Tumor Markers, Biological; EC 1.3.5.1/SDHB protein, human; EC 1.3.99.1/Succinate Dehydrogenase

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