Document Detail

A micro-radioimmunoassay for the measurement of intracellular cAMP.
MedLine Citation:
PMID:  7689853     Owner:  NLM     Status:  MEDLINE    
We have developed a rapid and sensitive micro-radioimmunoassay for the detection of cellular cAMP. The assay employs a simple method for preparing cellular lysates that requires as few as 500 cells per data point. Cellular lysates are incubated with cAMP-specific antibody complexes and [125I]cAMP in 96-well plates, harvested onto glass fiber filters and analyzed using a commercial autoradiograph imaging system. This method provides significant savings in reagents and may be particularly useful in situations where only a limited number of cells are available.
W Berg; P V Hornbeck
Related Documents :
15836763 - Early- and late-phase bronchoconstriction, airway hyper-reactivity and cell influx into...
6285993 - Alamethicin or detergent permeabilization of the cell membrane as a tool for adenylate ...
15846363 - Activity probe for in vivo profiling of the specificity of proteasome inhibitor bortezo...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  BioTechniques     Volume:  15     ISSN:  0736-6205     ISO Abbreviation:  BioTechniques     Publication Date:  1993 Jul 
Date Detail:
Created Date:  1993-10-04     Completed Date:  1993-10-04     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8306785     Medline TA:  Biotechniques     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  56-9     Citation Subset:  IM    
Department of Medicine, University of Maryland Medical School, Baltimore 21201.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
1-Methyl-3-isobutylxanthine / pharmacology
Cholera Toxin / pharmacology
Cyclic AMP / analysis*
Lymphoma, B-Cell / chemistry
Radioimmunoassay / methods*
Tumor Cells, Cultured
Reg. No./Substance:
28822-58-4/1-Methyl-3-isobutylxanthine; 60-92-4/Cyclic AMP; 9012-63-9/Cholera Toxin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Selective in vitro expansion of HLA class I-restricted HIV-1 Gag-specific CD8+ T cells: cytotoxic T-...
Next Document:  Crossed aphasia in a dextral patient--a case of sensory aphasia due to a right hemispheric lesion