| A micro-radioimmunoassay for the measurement of intracellular cAMP. | |
MedLine Citation:
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PMID: 7689853 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have developed a rapid and sensitive micro-radioimmunoassay for the detection of cellular cAMP. The assay employs a simple method for preparing cellular lysates that requires as few as 500 cells per data point. Cellular lysates are incubated with cAMP-specific antibody complexes and [125I]cAMP in 96-well plates, harvested onto glass fiber filters and analyzed using a commercial autoradiograph imaging system. This method provides significant savings in reagents and may be particularly useful in situations where only a limited number of cells are available. |
Authors:
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W Berg; P V Hornbeck |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: BioTechniques Volume: 15 ISSN: 0736-6205 ISO Abbreviation: BioTechniques Publication Date: 1993 Jul |
Date Detail:
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Created Date: 1993-10-04 Completed Date: 1993-10-04 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8306785 Medline TA: Biotechniques Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 56-9 Citation Subset: IM |
Affiliation:
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Department of Medicine, University of Maryland Medical School, Baltimore 21201. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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1-Methyl-3-isobutylxanthine
/
pharmacology Cholera Toxin / pharmacology Cyclic AMP / analysis* Humans Lymphoma, B-Cell / chemistry Microchemistry Radioimmunoassay / methods* Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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28822-58-4/1-Methyl-3-isobutylxanthine; 60-92-4/Cyclic AMP; 9012-63-9/Cholera Toxin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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