Document Detail


A micro-radioimmunoassay for the measurement of intracellular cAMP.
MedLine Citation:
PMID:  7689853     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have developed a rapid and sensitive micro-radioimmunoassay for the detection of cellular cAMP. The assay employs a simple method for preparing cellular lysates that requires as few as 500 cells per data point. Cellular lysates are incubated with cAMP-specific antibody complexes and [125I]cAMP in 96-well plates, harvested onto glass fiber filters and analyzed using a commercial autoradiograph imaging system. This method provides significant savings in reagents and may be particularly useful in situations where only a limited number of cells are available.
Authors:
W Berg; P V Hornbeck
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  BioTechniques     Volume:  15     ISSN:  0736-6205     ISO Abbreviation:  BioTechniques     Publication Date:  1993 Jul 
Date Detail:
Created Date:  1993-10-04     Completed Date:  1993-10-04     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8306785     Medline TA:  Biotechniques     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  56-9     Citation Subset:  IM    
Affiliation:
Department of Medicine, University of Maryland Medical School, Baltimore 21201.
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MeSH Terms
Descriptor/Qualifier:
1-Methyl-3-isobutylxanthine / pharmacology
Cholera Toxin / pharmacology
Cyclic AMP / analysis*
Humans
Lymphoma, B-Cell / chemistry
Microchemistry
Radioimmunoassay / methods*
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
28822-58-4/1-Methyl-3-isobutylxanthine; 60-92-4/Cyclic AMP; 9012-63-9/Cholera Toxin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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