Document Detail


A micro circulation system with a dialysis part for bioassays evaluating anticancer activity and retention.
MedLine Citation:
PMID:  23249269     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Medicines are distributed to the whole body and excreted over time. A micromodel of the circulation-excretion system was developed to mimic these processes. This system comprised a dialysis part, a micro peristaltic pump, and a target tissue. This micro circulation system was created on a microchip composed of a glass slide and polydimethylsiloxane (PDMS) sheets with microchannels fabricated by photolithography. A dialysis membrane was settled between 2 channels to form the dialysis part, and a pneumatic peristaltic pump was used to make the solution flow. The excretion and half-life of solute substances absorbed to albumin were changed according to their affinity to the protein. MCF-7 human breast cancer cells were cultured as target cells for drug samples, and the activities of anticancer agents were assayed using our system. Our data demonstrated that the anticancer activity of docetaxel or thio-TEPA could be assayed on the micro circulation-excretion chip. This system may allow for reduced consumption of cells and reagents compared to those required for conventional in-vitro bioassay systems.
Authors:
Yuki Imura; Etsuro Yoshimura; Kiichi Sato
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-19
Journal Detail:
Title:  Analytical chemistry     Volume:  -     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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