Document Detail


miRNA-processing enzyme Dicer is necessary for cardiac outflow tract alignment and chamber septation.
MedLine Citation:
PMID:  20018673     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
MicroRNAs (miRNAs) have previously been implicated in a number of developmental processes, including development of the ventricular myocardium of the heart. To determine what, if any, additional roles miRNAs play in cardiogenesis, we deleted the miRNA-processing enzyme Dicer specifically in the developing murine heart. Embryos lacking cardiac Dicer lived longer than reported in previous studies using different alleles to remove cardiac Dicer activity and displayed a highly penetrant phenotype of double outlet right ventricle with a concurrent ventricular septal defect. Before the defect's onset, Pitx2c and Sema3c, both required for outflow tract morphogenesis, were up-regulated in Dicer-deficient hearts. Interestingly, mesenchymal apoptosis in the outflow tract normally required for outflow tract alignment was greatly decreased in the mutants, likely contributing directly to the observed phenotype. In sum, we demonstrate here a specific developmental process, that of outflow tract morphogenesis, being hindered by the deletion of miRNAs during cardiogenesis.
Authors:
Ankur Saxena; Clifford J Tabin
Related Documents :
16336433 - Perimembranous and muscular ventricular septal defects--morphology revisited in the era...
12840243 - Percutaneous occlusion of the patent ductus arteriosus with the amplatzer device for at...
469443 - Cardiac electrophysiologic effects of etafenone hydrochloride (dialicor), a new antiarr...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2009-12-14
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  107     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-01-18     Completed Date:  2010-03-09     Revised Date:  2014-09-17    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  87-91     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Aorta / anatomy & histology,  embryology
Cell Death / physiology
Embryo, Mammalian / anatomy & histology,  physiology
Gene Deletion
Heart* / anatomy & histology,  embryology
Homeodomain Proteins / genetics,  metabolism
Mice
MicroRNAs / genetics,  metabolism*
Morphogenesis / physiology*
Myocardium / enzymology*
Ribonuclease III / genetics,  metabolism*
Semaphorins / genetics,  metabolism
Transcription Factors / genetics,  metabolism
Grant Support
ID/Acronym/Agency:
HD047360/HD/NICHD NIH HHS; R01 HD047360/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Homeodomain Proteins; 0/MicroRNAs; 0/Nkx2-5 protein, mouse; 0/Semaphorins; 0/Transcription Factors; 0/semaphorin 3C protein, mouse; 184787-43-7/homeobox protein PITX2; EC 3.1.26.3/Ribonuclease III
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  VGLUT2 in dopamine neurons is required for psychostimulant-induced behavioral activation.
Next Document:  Involvement of the Toll-like receptor 4 pathway and use of TNF-alpha antagonists for treatment of th...