Document Detail


miRNA and mRNA expression profiling identifies members of the miR-200 family as potential regulators of epithelial-mesenchymal transition in pterygium.
MedLine Citation:
PMID:  23872359     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The current study investigates whether microRNA (miRNA) regulators of epithelial-mesenchymal transition (EMT), tissue fibrosis, and angiogenesis are differentially expressed in human primary pterygium. Genome-wide miRNA and mRNA expression profiling of paired pterygium and normal conjunctiva was performed in the context of conventional excision of pterygium with autotransplantation of conjunctiva (n = 8). Quantitative real time polymerase chain reaction (qRT-PCR) was used to validate the expression of key molecules previously detected by microarray. In pterygium, 25 miRNAs and 31 mRNAs were significantly differentially expressed by more than two-fold compared to normal conjunctiva. 14 miRNAs were up-regulated (miR-1246, -486, -451, -3172, -3175, -1308, -1972, -143, -211, -665, -1973, -18a, 143, and -663b), whereas 11 were down-regulated (miR-675, -200b-star, -200a-star, -29b, -200b, -210, -141, -31, -200a, -934, and -375). Unsupervised hierarchical cluster analysis demonstrated that members of the miR-200 family were coexpressed and down-regulated in pterygium. The molecular and cellular functions that were most significant to the miRNA data sets were cellular development, cellular growth and proliferation, and cellular movement. qRT-PCR confirmed the expression of 15 of the 16 genes tested and revealed that miR-429 was down-regulated by more than two-fold in pterygium. The concerted down-regulation of four members from both clusters of the miR-200 family (miR-200a/-200b/-429 and miR-200c/-141), which are known to regulate EMT, and up-regulation of the predicted target and mesenchymal marker fibronectin (FN1), suggest that EMT could potentially play a role in the pathogenesis of pterygium and might constitute promising new targets for therapeutic intervention in pterygium.
Authors:
David H Engelsvold; Tor P Utheim; Ole K Olstad; Pedro Gonzalez; Jon R Eidet; Torstein Lyberg; Anne-Marie S Trøseid; Darlene A Dartt; Sten Raeder
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Publication Detail:
Type:  Journal Article     Date:  2013-07-19
Journal Detail:
Title:  Experimental eye research     Volume:  115     ISSN:  1096-0007     ISO Abbreviation:  Exp. Eye Res.     Publication Date:  2013 Oct 
Date Detail:
Created Date:  2013-10-08     Completed Date:  2013-12-13     Revised Date:  2014-11-01    
Medline Journal Info:
Nlm Unique ID:  0370707     Medline TA:  Exp Eye Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  189-98     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Elsevier Ltd. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Autografts
Cell Proliferation
Conjunctiva / transplantation
Epithelial-Mesenchymal Transition / genetics*
Female
Fibronectins / genetics
Fibrosis
Gene Expression Profiling
Gene Expression Regulation / physiology*
Humans
Male
MicroRNAs / genetics*
Middle Aged
Pterygium / genetics*,  surgery
RNA, Messenger / genetics*
Real-Time Polymerase Chain Reaction
Grant Support
ID/Acronym/Agency:
R01 EY022415/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/ED-B fibronectin protein, human; 0/Fibronectins; 0/MIRN200 microRNA, human; 0/MicroRNAs; 0/RNA, Messenger

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