Document Detail

miR-381, a novel intrinsic WEE1 inhibitor, sensitizes renal cancer cells to 5-FU by upregulationof Cdc2 activities in 786-O.
MedLine Citation:
PMID:  23816136     Owner:  NLM     Status:  Publisher    
BACKGROUND: Few researches on increase of chemotherapy sensitivity by microRNA (miRNA) werereported. We aim to investigate exact role of miR-381 in chemotherapy sensitivity of 5-fluorouracil (5-FU) inrenal cancer cells.METHODS: We investigated the cell survival, cell-cycle and apoptosis of 786-O and HK-2 cells treated withmiR-381 and 5-FU. IC50 of 5-FU was calculated. To study apoptosis and G2/M arrest, we determined pHH3, mitotic index and caspase-3/7 activity.
RESULTS: We showed that miR-381 combined with 5-FU inhibited proliferation and potentiated the antitumourefficacies of 5-FU at tolerated concentration in vitro. miR-381 combined with 5-FU led to Cd c2activation, mitotic catastrophe, and cell apoptosis through inhibitory WEE1. WEE1 was also validated as the direct target of miR-381. IC50 of 5-FU decreased significantly in the presence of miR-381.CONCLUSION: miR-381 increases sensitivity of 786-O cells to 5-FU by inhibitory WEE1 and increase of Cd c2activity.
Binghai Chen; Lujing Duan; Guangming Yin; Jing Tan; Xianzhen Jiang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-5-7
Journal Detail:
Title:  Journal of chemotherapy (Florence, Italy)     Volume:  -     ISSN:  1973-9478     ISO Abbreviation:  J Chemother     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-7-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8907348     Medline TA:  J Chemother     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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