Document Detail


The miR-30 family microRNAs confer epithelial phenotype to human pancreatic cells.
MedLine Citation:
PMID:  21099261     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Epithelial-to-mesenchymal transition is a phenomenon necessary for embryonic development and also seen during certain pathological conditions.  We show here for the first time that reduction in miR-30 family microRNAs, is responsible for mesenchymal transition of primary cultures of human pancreatic epithelial cells.  We found that miR-30 family microRNAs target mesenchymal gene transcripts and maintain them in a translationally inactive state.  Forced depletion using miR-30 family specific anti-miRs leads to mesenchymal transition while ectopic overexpression maintains the epithelial phenotype.  We also show that miR-30 family microRNAs increase in abundance during differentiation of pancreatic islet-derived mesenchymal cells into hormone-producing islet-like cell aggregates.  Our studies in human adult diseased pancreas also demonstrate that miR-30 family microRNAs are expressed at lower abundance in fibrotic lesions during pancreatitis.  Together, our data confirm that miR-30 family microRNAs form a part of the regulatory signaling events involved in cellular response of pancreatic epithelial cells during mesenchymal transition.
Authors:
Mugdha V Joglekar; Deepak Patil; Vinay M Joglekar; G V Rao; D Nageshwar Reddy; Sasikala Mitnala; Yogesh Shouche; Anandwardhan A Hardikar
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Islets     Volume:  1     ISSN:  1938-2022     ISO Abbreviation:  Islets     Publication Date:    2009 Sep-Oct
Date Detail:
Created Date:  2010-11-24     Completed Date:  2011-03-31     Revised Date:  2014-10-09    
Medline Journal Info:
Nlm Unique ID:  101495366     Medline TA:  Islets     Country:  United States    
Other Details:
Languages:  eng     Pagination:  137-47     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Base Sequence
Cell Dedifferentiation / genetics
Cell Differentiation / genetics
Cells, Cultured
Cluster Analysis
Epithelial Cells / cytology,  metabolism*,  physiology*
Fetus / cytology,  metabolism
Gene Expression Profiling
Gene Expression Regulation, Developmental
Humans
Mesenchymal Stromal Cells / metabolism,  physiology
MicroRNAs / genetics,  metabolism,  physiology*
Microarray Analysis
Models, Biological
Multigene Family / physiology
Pancreas / cytology*,  metabolism*
Phenotype
Chemical
Reg. No./Substance:
0/MIRN30 microRNA, human; 0/MicroRNAs
Comments/Corrections
Comment In:
Islets. 2009 Nov-Dec;1(3):283-5   [PMID:  21099286 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Downregulation of ZnT8 expression in pancreatic ?-cells of diabetic mice.
Next Document:  Physiological significance of SK4 channels in pancreatic ?-cell oscillations.