Document Detail

miR-27a regulates the growth, colony formation and migration of pancreatic cancer cells by targeting Sprouty2.
MedLine Citation:
PMID:  20638779     Owner:  NLM     Status:  MEDLINE    
MicroRNAs are short regulatory RNAs. A growing body of data implicates altered miRNA participate in the development of cancers and miR-27a is abnormally upregulated in several types of cancers identified as an oncogene. Although overexpressed in pancreatic adenocarcinoma, the oncogenic role of miR-27a has not yet been reported. In this study, we showed that inhibition of miR-27a suppressed the growth, colony formation and migration of pancreatic cancer cells. By using a reporter-screening assay, we discovered that the 3'UTR of Sprouty2 (Spry2) carried a putative miR-27a binding site. Furthermore, the Spry2 protein, which has a low expression level in pancreatic adenocarcinoma, was upregulated by transfection with a miR-27a inhibitor. The data reported here are the first to indicate that miR-27a plays an oncogenic role by targeting Spry2 and modulating the malignant, biological behavior of pancreatic cancer cells. This suggests the potential for miR-27a to be used as a target in the diagnosis and treatment of pancreatic adenocarcinoma.
Yihui Ma; Shuangni Yu; Wugan Zhao; Zhaohui Lu; Jie Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-07-17
Journal Detail:
Title:  Cancer letters     Volume:  298     ISSN:  1872-7980     ISO Abbreviation:  Cancer Lett.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-10-18     Completed Date:  2010-11-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  150-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.
Department of Pathology, Peking Union Medical College Hospital, People's Republic of China.
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MeSH Terms
3' Untranslated Regions / genetics
Adenocarcinoma / genetics,  metabolism,  pathology
Binding Sites / genetics
Blotting, Western
Cell Line
Cell Line, Tumor
Cell Movement*
Cell Proliferation*
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Intracellular Signaling Peptides and Proteins / genetics*,  metabolism
Luciferases / genetics,  metabolism
MicroRNAs / genetics*,  metabolism
Oligonucleotide Array Sequence Analysis
Pancreatic Neoplasms / genetics,  metabolism,  pathology
Reverse Transcriptase Polymerase Chain Reaction
Tumor Stem Cell Assay
Reg. No./Substance:
0/3' Untranslated Regions; 0/Intracellular Signaling Peptides and Proteins; 0/MIRN27 microRNA, human; 0/MicroRNAs; 0/SPRY2 protein, human; EC 1.13.12.-/Luciferases

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