Document Detail


miR-27a regulates the growth, colony formation and migration of pancreatic cancer cells by targeting Sprouty2.
MedLine Citation:
PMID:  20638779     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
MicroRNAs are short regulatory RNAs. A growing body of data implicates altered miRNA participate in the development of cancers and miR-27a is abnormally upregulated in several types of cancers identified as an oncogene. Although overexpressed in pancreatic adenocarcinoma, the oncogenic role of miR-27a has not yet been reported. In this study, we showed that inhibition of miR-27a suppressed the growth, colony formation and migration of pancreatic cancer cells. By using a reporter-screening assay, we discovered that the 3'UTR of Sprouty2 (Spry2) carried a putative miR-27a binding site. Furthermore, the Spry2 protein, which has a low expression level in pancreatic adenocarcinoma, was upregulated by transfection with a miR-27a inhibitor. The data reported here are the first to indicate that miR-27a plays an oncogenic role by targeting Spry2 and modulating the malignant, biological behavior of pancreatic cancer cells. This suggests the potential for miR-27a to be used as a target in the diagnosis and treatment of pancreatic adenocarcinoma.
Authors:
Yihui Ma; Shuangni Yu; Wugan Zhao; Zhaohui Lu; Jie Chen
Related Documents :
20848249 - Activation of the pi3k/akt pathway mediates bone morphogenetic protein 2-induced invasi...
18090159 - Kras mutation analysis of fine needle aspirate under eus guidance facilitates risk stra...
16421239 - A pooled analysis of second primary pancreatic cancer.
21594579 - Novel arf/p53-independent senescence pathways in cancer repression.
7554069 - Chemopreventive activity of oltipraz against n-nitrosobis(2-oxopropyl)amine (bop)-induc...
2424109 - The role of biliary obstruction in the pathogenesis of acute pancreatitis in the opossum.
1606339 - Anaphylactoid reaction to intravenous acetylcysteine associated with electrocardiograph...
11231939 - Racial and ethnic colorectal cancer patterns affect the cost-effectiveness of colorecta...
22202009 - Mirnas are stable in colorectal cancer archival tissue blocks.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-07-17
Journal Detail:
Title:  Cancer letters     Volume:  298     ISSN:  1872-7980     ISO Abbreviation:  Cancer Lett.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-10-18     Completed Date:  2010-11-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  150-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.
Affiliation:
Department of Pathology, Peking Union Medical College Hospital, People's Republic of China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
3' Untranslated Regions / genetics
Adenocarcinoma / genetics,  metabolism,  pathology
Binding Sites / genetics
Blotting, Western
Cell Line
Cell Line, Tumor
Cell Movement*
Cell Proliferation*
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry
Intracellular Signaling Peptides and Proteins / genetics*,  metabolism
Luciferases / genetics,  metabolism
MicroRNAs / genetics*,  metabolism
Oligonucleotide Array Sequence Analysis
Pancreatic Neoplasms / genetics,  metabolism,  pathology
Reverse Transcriptase Polymerase Chain Reaction
Tumor Stem Cell Assay
Chemical
Reg. No./Substance:
0/3' Untranslated Regions; 0/Intracellular Signaling Peptides and Proteins; 0/MIRN27 microRNA, human; 0/MicroRNAs; 0/SPRY2 protein, human; EC 1.13.12.-/Luciferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Activated Notch signaling is required for hepatitis B virus X protein to promote proliferation and s...
Next Document:  Cryptotanshinone sensitizes DU145 prostate cancer cells to Fas(APO1/CD95)-mediated apoptosis through...