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miR-203b: a novel regulator of MyoD expression in tilapia skeletal muscle.
MedLine Citation:
PMID:  23038733     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
MyoD is one of the helix-loop-helix proteins regulating muscle-specific gene expression in tilapia. Tight regulation of MyoD protein level is necessary for the precise regulation of skeletal muscle development. MicroRNAs (miRNAs) are a class of regulatory RNAs that post-transcriptionally regulate gene expression. Increasing evidences have suggested that miRNAs play an important role in regulating skeletal muscle development. We reasoned that MyoD expression may be regulated by miRNAs. Bioinformatics prediction identify a putative miR-203b target site in the 3'-UTR of MyoD gene. Interestingly, miR-203b expression is negatively correlated is negatively correlated with MyoD expression. miR-203b suppression leads to a significant increase in MyoD expression, thereby activating MyoD downstream gene. 3'-UTR luciferase reporter assay further verifies the direct interaction between miR-203b and MyoD. Taken together, our studies reveal a novel molecular mechanism in which miRNA participates in transcriptional circuits that regulates gene expression in tilapia skeletal muscle.
Authors:
Biao Yan; Li-Hui Zhao; Jin-Tao Guo; Jin-Liang Zhao
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-4
Journal Detail:
Title:  The Journal of experimental biology     Volume:  -     ISSN:  1477-9145     ISO Abbreviation:  J. Exp. Biol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0243705     Medline TA:  J Exp Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Shanghai Ocean University, China.
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