Document Detail


miR-181a sensitizes a multidrug-resistant leukemia cell line K562/A02 to daunorubicin by targeting BCL-2.
MedLine Citation:
PMID:  22285729     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The aim of this study was to investigate whether miR-181a could modulate the sensitivity of the leukemia drug-resistant cell line K562/A02 to the chemotherapeutic agent daunorubicin (DNR), and explore the mechanism of miR-181a on the DNR sensitivity of K562/A02 cells. MicroRNA microarray and stem-loop reverse transcription-polymerase chain reaction were used to detect the expression of miR-181a. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide  assay was performed to quantify the effect of miR-181a on K562 cells growth and viability. Apoptotic cells were quantitatively detected using Annexin V/FITC and PI apoptosis detection kit. BCL-2 protein expression was measured by western blot. Luciferase reporter vector with the putative BCL-2 3' untranslated region was constructed to explore whether BCL-2 was a direct target gene of miR-181a. BCL-2 siRNA was transfected into the cell to explore the relationship between BCL-2 and DNR resistance. The miR-181a expression level was lower in the K562/A02 cells than in the K562 cells (P< 0.05). K562 cells that were transfected with miR-181a inhibitor had a significantly higher survival than K562 cells, and K562/A02 cells that were transfected with the miR-181a mimic had a significantly lower survival than K562/A02 cells (P< 0.05). miR-181a could enhance DNR-induced apoptosis in K562/A02 cells. BCL-2 siRNA transfected K562/A02 cells had decreased survival compared with the K562/A02 control group. In conclusion, miR-181a could play a role in the development of DNR resistance in K562/A02 cells and the over-expression of miR-181a could sensitize K562/A02 cells to DNR by targeting BCL-2.
Authors:
Hao Li; Lulu Hui; Wenlin Xu
Related Documents :
7813009 - Glycogen synthase kinase 3 regulates cell fate in dictyostelium.
1277179 - Differential inhibition of embryonic cell aggregation by cultured human cells with "mal...
8901579 - Myosin light chain kinase (mlck) gene disruption in dictyostelium: a role for mlck-a in...
14607429 - Cell behavior and cell-cell communication during fruiting body morphogenesis in myxococ...
16885229 - Prefibrillar amyloid aggregates could be generic toxins in higher organisms.
22859339 - Silencing of snx1 by sirna stimulates the ligand-induced endocytosis of egfr and increa...
8687479 - Characterization of the catecholamine extraneuronal uptake2 carrier in human glioma cel...
12031259 - Labile intracellular zinc is associated with 3t3 cell growth.
23155249 - Suppression of cell invasion and migration by propofol are involved in down-regulating ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-26
Journal Detail:
Title:  Acta biochimica et biophysica Sinica     Volume:  -     ISSN:  1745-7270     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101206716     Medline TA:  Acta Biochim Biophys Sin (Shanghai)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Central Laboratory, The Affiliated People's Hospital, Jiangsu University, Zhenjiang 212001, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  RNA interference in vitro and in vivo using DsiRNA targeting the nucleocapsid N mRNA of human metapn...
Next Document:  Low microRNA-199a expression in human amniotic epithelial cell feeder layers maintains human-induced...