| miR-15b and miR-16 are implicated in activation of the rat hepatic stellate cell: An essential role for apoptosis. | |
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MedLine Citation:
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PMID: 19232449 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND/AIMS: To reveal the microRNA (miRNA) expression profile and related roles in rat HSCs during activation. METHODS: miRNA expression profiling was analyzed in quiescent and in culture-activated HSCs by microarray. The differentially expressed miRNAs, as verified by RT-PCR, were subjected to gene ontology (GO) analysis. Furthermore, the effects of miR-16 and miR-15b on the apoptosis of activated HSCs were investigated by Hoechst 33258, TUNEL staining and annexin-V/PI labeling flow cytometry. The underlying mechanism related to Bcl-2 and caspases was assessed. RESULTS: The upregulated and downregulated miRNAs in activated HSCs were 12 miRNAs and 9 miRNAs, respectively. The differential expression of miR-16, -15b, -122, -138, -143, and -140 was validated. High-enrichment GOs containing apoptosis-related targeted genes and miRNA-gene networks characterized by Bcl-2, which was targeted by the miR-15/16 family, uncovered the critical role of miR-16 and miR-15b in apoptosis. Restoring the intracellular miRNAs by miR-16 and miR-15b administration greatly reduced Bcl-2, and increased the expression of caspases 3, 8, and 9. Significantly elevated rates of apoptosis were then induced in activated HSCs. CONCLUSIONS: The activation of HSCs relate to 21 miRNAs. Among these, mir-15b and miR-16 may be essential for apoptosis by targeting Bcl-2 and the caspase signaling pathway. |
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Authors:
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Can-Jie Guo; Qin Pan; Ding-Guo Li; Hua Sun; Bo-Wei Liu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-02-02 |
Journal Detail:
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Title: Journal of hepatology Volume: 50 ISSN: 1600-0641 ISO Abbreviation: J. Hepatol. Publication Date: 2009 Apr |
Date Detail:
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Created Date: 2009-03-16 Completed Date: 2009-07-29 Revised Date: 2012-08-24 |
Medline Journal Info:
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Nlm Unique ID: 8503886 Medline TA: J Hepatol Country: England |
Other Details:
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Languages: eng Pagination: 766-78 Citation Subset: IM |
Affiliation:
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Digestive Disease Laboratory and Department of Gastroenterology, School of Medicine, Shanghai Jiaotong University, Xinhua Hospital, No. 1665 Kongjiang Road, Shanghai 200092, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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genetics Animals Apoptosis / physiology* DNA Primers Desmin / genetics Down-Regulation Flow Cytometry Hepatic Stellate Cells / cytology, physiology* In Situ Nick-End Labeling Male MicroRNAs / genetics* Microarray Analysis Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction Up-Regulation |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/DNA Primers; 0/Desmin; 0/MicroRNAs; 0/smooth muscle actin, rat |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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