Document Detail

miR-143 is critical for the formation of primordial follicles in mice.
MedLine Citation:
PMID:  23276944     Owner:  NLM     Status:  MEDLINE    
Although microRNAs (miRNAs) have been implicated in fine-tuning gene networks, the roles of mmu-mir-143 (miR-143) in mammalian ovary development have not been studied in vitro. We investigated the expression and function of miR-143 in the mouse ovary during primordial follicle formation. Real-time polymerase chain reaction analysis showed that miR-143 expression increased during primordial follicle formation from 15.5 days post-coitus to 4 days post-partum. miR-143 was located in pregranulosa cells by in situ hybridization. To study the function of miR-143 in primordial follicle formation we established an electroporation transfection model in vitro that allowed miR-143 expression to be efficiently upregulated and inhibited in cultured ovaries. Further studies showed that miR-143 inhibited the formation of primordial follicles by suppressing pregranulosa cell proliferation and downregulating the expression of genes related to the cell cycle. These findings suggest that miR-143 is critical for the formation of primordial follicles and regulates ovarian development and function.
Jianfang Zhang; Xiaowen Ji; Doudou Zhou; Yueqin Li; Junkai Lin; Jiali Liu; Haoshu Luo; Sheng Cui
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-01-01
Journal Detail:
Title:  Frontiers in bioscience (Landmark edition)     Volume:  18     ISSN:  1093-4715     ISO Abbreviation:  Front Biosci (Landmark Ed)     Publication Date:  2013  
Date Detail:
Created Date:  2013-01-01     Completed Date:  2013-06-12     Revised Date:  2013-07-29    
Medline Journal Info:
Nlm Unique ID:  101612996     Medline TA:  Front Biosci (Landmark Ed)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  588-97     Citation Subset:  IM    
State Key Laboratory for Agro-biotechnology, College of Biological Sciences, China Agricultural University, No.2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, P.R. China.
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MeSH Terms
Cell Cycle Proteins / biosynthesis,  drug effects
MicroRNAs / biosynthesis,  physiology*
Ovarian Follicle / drug effects,  physiology*
Ovary / growth & development
Reg. No./Substance:
0/Cell Cycle Proteins; 0/MicroRNAs; 0/Mirn143 microRNA, mouse

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