Document Detail

miR-141 as a regulator of the mitochondrial phosphate carrier (Slc25a3) in the type 1 diabetic heart.
MedLine Citation:
PMID:  23034391     Owner:  NLM     Status:  MEDLINE    
Dysfunctional mitochondria are central in the pathogenesis of diabetic cardiomyopathy. Mitochondrial proteomic alterations resulting from diabetes mellitus have been reported although the mechanisms driving changes in proteomic signatures are unknown. microRNAs (miRNAs) have been considered as potential regulators of proteins. The goal of this study was to determine whether miRNAs play a role in diabetes-induced mitochondrial proteomic alterations. Quanitative RT-PCR miRNA screening in diabetic mice, 5 wk following multiple low-dose streptozotocin treatment was associated with alteration in the expression of 29 miRNAs in the diabetic heart compared with control. Among those miRNAs upregulated in the diabetic heart was miR-141 (P < 0.002). miRNA target prediction analyses identified miR-141 as a potential regulator of the inner mitochondrial membrane phosphate transporter, solute carrier family 25 member 3 (Slc25a3), which provides inorganic phosphate to the mitochondrial matrix and is essential for ATP production. With the use of a luciferase reporter construct with a Slc25a3 3'-untranslated region (UTR) target sequence, overexpression of miR-141 downregulated luciferase activity levels confirming miR-141/Slc25a3 3'-UTR binding. miR-141 overexpression in HL-1 cells elicited a decrease in Slc25a3 protein content, ATP production and a decrease in ATP synthase activity, similar to the diabetic phenotype (P < 0.05, for both). Diabetic interfibrillar mitochondria (IFM) displayed decreased Slc25a3 protein content, which was inversely correlated with increased miR-141 expression. Further, diabetic IFM ATP synthase activity was also decreased (P < 0.05). Together these results indicate that miR-141 can regulate Slc25a3 protein expression in the diabetic heart. Further, diabetes-induced miRNA changes may influence mitochondrial proteomes and functional processes such as mitochondrial ATP production.
Walter A Baseler; Dharendra Thapa; Rajaganapathi Jagannathan; Erinne R Dabkowski; Tara L Croston; John M Hollander
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-10-03
Journal Detail:
Title:  American journal of physiology. Cell physiology     Volume:  303     ISSN:  1522-1563     ISO Abbreviation:  Am. J. Physiol., Cell Physiol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-17     Completed Date:  2013-02-26     Revised Date:  2013-12-18    
Medline Journal Info:
Nlm Unique ID:  100901225     Medline TA:  Am J Physiol Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  C1244-51     Citation Subset:  IM    
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MeSH Terms
Diabetes Mellitus, Experimental / metabolism*
Diabetes Mellitus, Type 1 / metabolism*
Diabetic Cardiomyopathies / metabolism*
MicroRNAs / metabolism*
Mitochondria, Heart / metabolism
Mitochondrial Proteins / biosynthesis*
Mitochondrial Proton-Translocating ATPases / metabolism
Phosphate Transport Proteins / biosynthesis*
Grant Support
Reg. No./Substance:
0/MicroRNAs; 0/Mirn141 microRNA, mouse; 0/Mitochondrial Proteins; 0/Phosphate Transport Proteins; 0/SLC25A3 protein, human; EC 3.6.3.-/Mitochondrial Proton-Translocating ATPases

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