| The methylene blue colorimetric microassay for determining cell line response to growth factors. | |
| | |
MedLine Citation:
|
PMID: 22359207 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
The validity of the methylene blue colorimetric microassay for determining the response of monolayers of human ovarian tumour cell lines to different growth factors was investigated. Linearity of the relationship between cell density and optical density was confirmed for each cell line (r=0.989-0.999,p<0.001), and when initial cell density was optimised to give exponential growth over the assay period, differences in response to medium supplements were obvious. The response of target cells to growth factors, obtained using the methylene blue assay, were compared with, and found to parallel, previously documented responses obtained non-colorimetrically. Thus Mink lung epithelial cells (MLEC) were inhibited by TGβ (Holleyet al., 1983), EGF had an inhibitory effect on A431 cells (Gill & Lazar, 1981; Barnes, 1982), and the mesothelial cell line showed a proliferative response to EGF and hydrocortisone (Connell and Rheinwald, 1983).The methylene blue colorimetric microssay was found to be a simple, reliable, sensitive method with low variability, for determining the response of cultured cells to growth factors. |
| | |
Authors:
|
M F Dent; L Hubbold; H Radford; A P Wilson |
Related Documents
:
|
22890317 - Aneuploid human colonic epithelial cells are sensitive to aicar-induced growth inhibiti... 9350657 - Intracellular transport, cell-surface exposure and release of recombinant tamm-horsfall... 261527 - Cell surface components of intestinal epithelial cells and their relationship to cellul... 6815277 - Cell sorting using a universally applicable affinity chromatography matrix: solid-phase... 9736667 - Postnatal development of type i and type ii hair cells in the mouse utricle: acquisitio... 1901867 - Endocytosis and intracellular transport of the glycolipid-binding ligand shiga toxin in... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Cytotechnology Volume: 17 ISSN: 0920-9069 ISO Abbreviation: Cytotechnology Publication Date: 1995 Feb |
Date Detail:
|
Created Date: 2012-02-23 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8807027 Medline TA: Cytotechnology Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 27-33 Citation Subset: - |
Affiliation:
|
Oncology Research Laboratory, Derby City General Hospital, Uttoxeter Road, DE22 3NE, Derby, UK. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Strong buffering capacity of insect cells. Implications for the baculovirus expression system.
Next Document: Changes in metabolism and hormone trafficking during exposure of endocrine cells to elevated ammoniu...