Document Detail


The methylene blue colorimetric microassay for determining cell line response to growth factors.
MedLine Citation:
PMID:  22359207     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The validity of the methylene blue colorimetric microassay for determining the response of monolayers of human ovarian tumour cell lines to different growth factors was investigated. Linearity of the relationship between cell density and optical density was confirmed for each cell line (r=0.989-0.999,p<0.001), and when initial cell density was optimised to give exponential growth over the assay period, differences in response to medium supplements were obvious. The response of target cells to growth factors, obtained using the methylene blue assay, were compared with, and found to parallel, previously documented responses obtained non-colorimetrically. Thus Mink lung epithelial cells (MLEC) were inhibited by TGβ (Holleyet al., 1983), EGF had an inhibitory effect on A431 cells (Gill & Lazar, 1981; Barnes, 1982), and the mesothelial cell line showed a proliferative response to EGF and hydrocortisone (Connell and Rheinwald, 1983).The methylene blue colorimetric microssay was found to be a simple, reliable, sensitive method with low variability, for determining the response of cultured cells to growth factors.
Authors:
M F Dent; L Hubbold; H Radford; A P Wilson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytotechnology     Volume:  17     ISSN:  0920-9069     ISO Abbreviation:  Cytotechnology     Publication Date:  1995 Feb 
Date Detail:
Created Date:  2012-02-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8807027     Medline TA:  Cytotechnology     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  27-33     Citation Subset:  -    
Affiliation:
Oncology Research Laboratory, Derby City General Hospital, Uttoxeter Road, DE22 3NE, Derby, UK.
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