| A methodological study of discontinuous Percoll-gradient separation of pancreatic islets from rats. | |
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MedLine Citation:
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PMID: 3310194 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A discontinuous Percoll gradient was used to separate pancreatic islets from rats. Adjusting the osmolality to 315 mosmol kg-1 H2O, working at 4 degrees C and filtration of the pancreatic digest prior to gradient separation were vital for securing an optimal separation. The gradient design was also critical. Thus, optimal results were obtained with gradient centrifugation at 800 g, and using a bottom gradient that maintained the tissue evenly distributed prior to centrifugation. No significant difference was observed in the insulin release from islets isolated manually and islets isolated on Percoll gradients. Neither was there any significant differences in the insulin release from Percoll-separated islets stimulated in solutions containing 25 mmol l-1 NaHCO3 and islets stimulated in solutions containing only 4.2 mmol l-1 NaHCO3. In conclusion, discontinuous-Percoll gradients can be used for isolation of pancreatic islets from rats. |
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Authors:
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B Vonen; J Florholmen; A K Giaever; P G Burhol |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Scandinavian journal of clinical and laboratory investigation Volume: 47 ISSN: 0036-5513 ISO Abbreviation: Scand. J. Clin. Lab. Invest. Publication Date: 1987 Sep |
Date Detail:
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Created Date: 1987-11-13 Completed Date: 1987-11-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0404375 Medline TA: Scand J Clin Lab Invest Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 415-20 Citation Subset: IM |
Affiliation:
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Institute of Clinical Medicine, University of Tromsø, Norway. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Separation / methods* Cells, Cultured Centrifugation, Density Gradient / methods Insulin / secretion Islets of Langerhans / physiology*, secretion Male Osmolar Concentration Rats Rats, Inbred Strains |
| Chemical | |
Reg. No./Substance:
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11061-68-0/Insulin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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