Document Detail


A method to study intracellular ice nucleation.
MedLine Citation:
PMID:  9675677     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The thermodynamics of intracellular ice nucleation are important in low-temperature biology for understanding and controlling the process of cell destruction by freezing. We have developed a new apparatus and technique for studying the physics of intracellular ice nucleation. Employing the principle of directional solidification in conjunction with light microscopy, we can generate information on the temperature at which ice nucleates intracellularly as a function of the thermal history the cells experience. The methodology is introduced, and results with primary prostatic cancer cells are described.
Authors:
K Tatsutani; B Rubinsky
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of biomechanical engineering     Volume:  120     ISSN:  0148-0731     ISO Abbreviation:  J Biomech Eng     Publication Date:  1998 Feb 
Date Detail:
Created Date:  1998-10-28     Completed Date:  1998-10-28     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7909584     Medline TA:  J Biomech Eng     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  27-31     Citation Subset:  IM    
Affiliation:
Department of Mechanical Engineering, University of California at Berkeley 94720, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Survival / physiology*
Cryopreservation*
Cryosurgery*
Humans
Ice*
Intracellular Fluid / physiology
Male
Prostatic Neoplasms
Thermodynamics
Tumor Cells, Cultured / pathology*
Grant Support
ID/Acronym/Agency:
R01CA56898/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Ice

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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