| A method to study intracellular ice nucleation. | |
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MedLine Citation:
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PMID: 9675677 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The thermodynamics of intracellular ice nucleation are important in low-temperature biology for understanding and controlling the process of cell destruction by freezing. We have developed a new apparatus and technique for studying the physics of intracellular ice nucleation. Employing the principle of directional solidification in conjunction with light microscopy, we can generate information on the temperature at which ice nucleates intracellularly as a function of the thermal history the cells experience. The methodology is introduced, and results with primary prostatic cancer cells are described. |
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Authors:
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K Tatsutani; B Rubinsky |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of biomechanical engineering Volume: 120 ISSN: 0148-0731 ISO Abbreviation: J Biomech Eng Publication Date: 1998 Feb |
Date Detail:
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Created Date: 1998-10-28 Completed Date: 1998-10-28 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 7909584 Medline TA: J Biomech Eng Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 27-31 Citation Subset: IM |
Affiliation:
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Department of Mechanical Engineering, University of California at Berkeley 94720, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Survival
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physiology* Cryopreservation* Cryosurgery* Humans Ice* Intracellular Fluid / physiology Male Prostatic Neoplasms Thermodynamics Tumor Cells, Cultured / pathology* |
| Grant Support | |
ID/Acronym/Agency:
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R01CA56898/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Ice |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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