| A method to determine the kinetics of multiple proteins in human infants with respiratory distress syndrome. | |
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MedLine Citation:
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PMID: 22526637 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We report a method to measure in vivo turnover of four proteins from sequential tracheal aspirates obtained from human newborn infants with respiratory distress syndrome using targeted proteomics. We detected enrichment for all targeted proteins approximately 3 h from the start of infusion of [5,5,5-(2)H(3)] leucine, secretion times that varied from 1.2 to 2.5 h, and half lives that ranged between 10 and 21 h. Complement factor B, a component of the alternative pathway of complement activation, had an approximately twofold-longer half-life than the other three proteins. In addition, the kinetics of mature and carboxy-terminal tryptic peptides from the same protein (surfactant protein B) were not statistically different (p = 0.49). |
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Authors:
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Michael S Bereman; Daniela M Tomazela; Hillary S Heins; Manuela Simonato; Paola E Cogo; Aaron Hamvas; Bruce W Patterson; F Sessions Cole; Michael J MacCoss |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2012-04-14 |
Journal Detail:
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Title: Analytical and bioanalytical chemistry Volume: 403 ISSN: 1618-2650 ISO Abbreviation: Anal Bioanal Chem Publication Date: 2012 Jun |
Date Detail:
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Created Date: 2012-06-11 Completed Date: 2012-10-01 Revised Date: 2013-05-13 |
Medline Journal Info:
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Nlm Unique ID: 101134327 Medline TA: Anal Bioanal Chem Country: Germany |
Other Details:
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Languages: eng Pagination: 2397-402 Citation Subset: IM |
Affiliation:
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Department of Genome Sciences, University of Washington, Seattle, WA 98195-5502, USA. mbereman@uw.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Complement Factor B / analysis, metabolism Humans Infant, Newborn Kinetics Mass Spectrometry / methods Molecular Sequence Data Protein Precursors / analysis, metabolism Proteins / analysis*, metabolism* Proteolipids / analysis, metabolism Proteomics / methods* Pulmonary Surfactant-Associated Protein B / analysis, metabolism Respiratory Distress Syndrome, Newborn / metabolism* Trachea / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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R01 DK069386/DK/NIDDK NIH HHS; R01 DK069386/DK/NIDDK NIH HHS; R01 HL065174/HL/NHLBI NIH HHS; R01 HL082747/HL/NHLBI NIH HHS; R01 HL082747/HL/NHLBI NIH HHS; T32 HG000035/HG/NHGRI NIH HHS; T32 HG000035/HG/NHGRI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Protein Precursors; 0/Proteins; 0/Proteolipids; 0/Pulmonary Surfactant-Associated Protein B; 0/surfactant protein B propeptide; EC 3.4.21.47/Complement Factor B |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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