Document Detail


A method to determine the kinetics of multiple proteins in human infants with respiratory distress syndrome.
MedLine Citation:
PMID:  22526637     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report a method to measure in vivo turnover of four proteins from sequential tracheal aspirates obtained from human newborn infants with respiratory distress syndrome using targeted proteomics. We detected enrichment for all targeted proteins approximately 3 h from the start of infusion of [5,5,5-(2)H(3)] leucine, secretion times that varied from 1.2 to 2.5 h, and half lives that ranged between 10 and 21 h. Complement factor B, a component of the alternative pathway of complement activation, had an approximately twofold-longer half-life than the other three proteins. In addition, the kinetics of mature and carboxy-terminal tryptic peptides from the same protein (surfactant protein B) were not statistically different (p = 0.49).
Authors:
Michael S Bereman; Daniela M Tomazela; Hillary S Heins; Manuela Simonato; Paola E Cogo; Aaron Hamvas; Bruce W Patterson; F Sessions Cole; Michael J MacCoss
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-04-14
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  403     ISSN:  1618-2650     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-06-11     Completed Date:  2012-10-01     Revised Date:  2013-07-22    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2397-402     Citation Subset:  IM    
Affiliation:
Department of Genome Sciences, University of Washington, Seattle, WA 98195-5502, USA. mbereman@uw.edu
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Complement Factor B / analysis,  metabolism
Humans
Infant, Newborn
Kinetics
Mass Spectrometry / methods
Molecular Sequence Data
Protein Precursors / analysis,  metabolism
Proteins / analysis*,  metabolism*
Proteolipids / analysis,  metabolism
Proteomics / methods*
Pulmonary Surfactant-Associated Protein B / analysis,  metabolism
Respiratory Distress Syndrome, Newborn / metabolism*
Trachea / metabolism*
Grant Support
ID/Acronym/Agency:
R01 DK069386/DK/NIDDK NIH HHS; R01 DK069386/DK/NIDDK NIH HHS; R01 GM103551/GM/NIGMS NIH HHS; R01 HL065174/HL/NHLBI NIH HHS; R01 HL082747/HL/NHLBI NIH HHS; R01 HL082747/HL/NHLBI NIH HHS; T32 HG000035/HG/NHGRI NIH HHS; T32 HG000035/HG/NHGRI NIH HHS
Chemical
Reg. No./Substance:
0/Protein Precursors; 0/Proteins; 0/Proteolipids; 0/Pulmonary Surfactant-Associated Protein B; 0/surfactant protein B propeptide; EC 3.4.21.47/Complement Factor B
Comments/Corrections

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