Document Detail

A method for studying rat embryonic metabolism by microcannulation of the vitelline circulation.
MedLine Citation:
PMID:  2606767     Owner:  NLM     Status:  MEDLINE    
A novel technique which involves the cannulation of the vitelline vessels of the 11.5 days rat embryo is described; this has allowed the introduction of both macromolecular and particulate substances directly into the embryo, thus bypassing the digestive and metabolic activity of the visceral yolk sac and facilitating the study of the fate of such substances within the embryo. The method does not affect the growth of the conceptus in vitro providing that inert solutions (e.g. azo blue dye in Hank's balanced salt solution) in volumes of less than 1.0 microliter are employed. When India ink is injected, the vitelline vessels and the intraembryonic vessels are seen to be extensively perfused. Approximately 80% of injected 125I-labelled polyvinylpyrrolidone is retained by the conceptus after injection, and it is distributed mainly in the embryo and exocoelomic cavity.
E P Mensah-Brown; M K Pratten; F Beck
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of anatomy     Volume:  163     ISSN:  0021-8782     ISO Abbreviation:  J. Anat.     Publication Date:  1989 Apr 
Date Detail:
Created Date:  1990-02-22     Completed Date:  1990-02-22     Revised Date:  2010-08-25    
Medline Journal Info:
Nlm Unique ID:  0137162     Medline TA:  J Anat     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  123-34     Citation Subset:  IM    
Department of Anatomy, University of Leicester, U.K.
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MeSH Terms
Blood Circulation / physiology
Catheterization / methods*
Embryo, Mammalian / blood supply,  metabolism*
Povidone-Iodine / diagnostic use
Vitelline Membrane / blood supply*,  physiology
Grant Support
//Wellcome Trust
Reg. No./Substance:

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