Document Detail


A method for slowing down depletion of myocardial energy reserves of the donor heart before transplantation.
MedLine Citation:
PMID:  2029656     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
One of the methods of donor heart protection against ischemia is a substantial lowering of temperature of the heart perfused with cardioplegic solution (CS). The achieved conservation of energetically rich compounds, however, does not guarantee the full restoration of heart function during reperfusion. Another possibility for heart preservation is repeated application of CS at 20 degrees C. This variant was tested in our experiments on isolated rat hearts perfused under constant pressure with the Krebs-Henseleit solution according to Langendorff. During global ischemia (180 min at 20 degrees C) we applied the St. Thomas Hospital CS lx or 4 x at 60 min intervals. During the ischemia, glycogen, ATP, lactate, Na+, K+ were assessed in the heart. The heart injury was monitored as the release of lactate dehydrogenase (LD) during the 60 min reperfusion. Repeated CS perfusion of the heart during ischemia lowers the contents of lactate and maintains ATP and glycogen content at elevated levels throughout ischemia. The improved condition of the heart after repeated CS application demonstrated as prevention of the gain of Na+ in the cells at the end of ischemia as well as after reperfusion. This was associated with reduced intracellular potassium depletion and LD release into the perfusate.
Authors:
H Vavrínková; M Tutterová; Z Vránová; M Vrána
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Bratislavské lekárske listy     Volume:  92     ISSN:  0006-9248     ISO Abbreviation:  Bratisl Lek Listy     Publication Date:    1991 Mar-Apr
Date Detail:
Created Date:  1991-06-20     Completed Date:  1991-06-20     Revised Date:  2009-11-11    
Medline Journal Info:
Nlm Unique ID:  0065324     Medline TA:  Bratisl Lek Listy     Country:  CZECHOSLOVAKIA    
Other Details:
Languages:  eng     Pagination:  150-4     Citation Subset:  IM    
Affiliation:
Institut klinické a experimentální medicíny, Praha, CSFR.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / metabolism
Animals
Cardioplegic Solutions / administration & dosage
Energy Metabolism*
Glycogen / metabolism
Heart Transplantation*
Lactates / metabolism
Lactic Acid
Myocardial Reperfusion*
Myocardium / metabolism*
Organ Preservation / methods*
Rats
Chemical
Reg. No./Substance:
0/Cardioplegic Solutions; 0/Lactates; 50-21-5/Lactic Acid; 56-65-5/Adenosine Triphosphate; 9005-79-2/Glycogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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