| A method for sequential excision biopsies of rat liver in an isolated perfused system. | |
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MedLine Citation:
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PMID: 1619983 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We describe a simple method of performing sequential excision biopsies during liver reperfusion in the isolated perfused rat liver. After hepatectomy, four ligatures (5.0 silk) tied with a slip knot are placed around the pedicles of: (1) the inferior and (2) the superior parts of the caudate lobe, as well as (3) the inferior and (4) the superior parts of the right lateral lobe. At the time of biopsy, the prepared 5.0 silk ties are tightened with sufficient force to occlude the vascular pedicle, preventing leakage of circulating perfusate. The procedure provides four biopsies of more than 350 mg each without alteration of perfusate transaminases and tissue ATP contents. The total tissue removed by this method comprises 20-25% of the whole liver weight. |
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Authors:
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P A Clavien; J R Sanabria; R Cywes; P R Harvey; S M Strasberg |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Liver Volume: 12 ISSN: 0106-9543 ISO Abbreviation: Liver Publication Date: 1992 Apr |
Date Detail:
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Created Date: 1992-08-05 Completed Date: 1992-08-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8200939 Medline TA: Liver Country: DENMARK |
Other Details:
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Languages: eng Pagination: 69-72 Citation Subset: IM |
Affiliation:
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Samuel Lunenfeld Research Institute, Toronto, Ontario, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biopsy / methods* Hepatectomy Liver / surgery* Male Organ Size Perfusion Rats Rats, Inbred Lew Rats, Inbred Strains |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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